| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00057395m |
| Family | GH32 |
| Protein Properties | Length: 582 Molecular Weight: 64356.1 Isoelectric Point: 9.1196 |
| Chromosome | Chromosome/Scaffold: 028396 Start: 3321 End: 7579 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 57 | 374 | 0 |
| HFQPPRNWINDPNAPMYYKGWYHLFYQYNPKGAVWGNIVWAHSVSRDLINWIALEPAIYPSIPSDQFGCWSGSATLLPDGTPAITYTGISRSDINYQVQN VAFPKNASDPLLREWVKPAELNPIAVPEGGINATQFRDPTTAWYADGHWRMLVGGVRGGARGLAFVYRSRDFRRWRRARKPLHSAATGMWECPDFFPVSG AGLENGLDTSEAGPKYVLKNSLDLTRYDYYTVGSYDRRRDRYVPDDPAGDERRRRYDYGNFYASKTFYDPAKRRRVLLGWANESDSVPDDKAKGWAGIHA IPRKIWLDPSGKQLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 582 Download |
| MGTPQRVLLA PWALLAVVAL QLAGASHVIH WSLEAEAAPP PSVPASIVSP LLKTGYHFQP 60 PRNWINDPNA PMYYKGWYHL FYQYNPKGAV WGNIVWAHSV SRDLINWIAL EPAIYPSIPS 120 DQFGCWSGSA TLLPDGTPAI TYTGISRSDI NYQVQNVAFP KNASDPLLRE WVKPAELNPI 180 AVPEGGINAT QFRDPTTAWY ADGHWRMLVG GVRGGARGLA FVYRSRDFRR WRRARKPLHS 240 AATGMWECPD FFPVSGAGLE NGLDTSEAGP KYVLKNSLDL TRYDYYTVGS YDRRRDRYVP 300 DDPAGDERRR RYDYGNFYAS KTFYDPAKRR RVLLGWANES DSVPDDKAKG WAGIHAIPRK 360 IWLDPSGKQL LQWPVEEVEK LRGKPVSVAG KVVKPGQHFE VTGLATYQAD VEVSFEVSSL 420 DRAEPFDAAY ANDAQKLCGV KGADAKGGVG PFGLWVLASA DLQEKTAVFF RVFKDGYGKP 480 KVLMCTDPTK SSLSPDLYKP TFAGFVDTDI SSGKITLRSL IDRSVVESFG AGGRTCILSR 540 VYPSIAVGKD AHLYVFNNGE ADIKVSHLTA WEMKKPLMNG A* |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| TIGR01322 | scrB_fam | 4.0e-42 | 54 | 389 | 356 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
| COG1621 | SacC | 3.0e-53 | 56 | 544 | 500 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
| cd08996 | GH32_B_Fructosidase | 5.0e-76 | 63 | 377 | 319 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
| pfam00251 | Glyco_hydro_32N | 2.0e-121 | 57 | 374 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
| smart00640 | Glyco_32 | 2.0e-163 | 57 | 533 | 485 | + Glycosyl hydrolases family 32. |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD02511.1 | 0 | 27 | 581 | 32 | 591 | cell wall invertase Incw1 [Zea mays] |
| GenBank | AAP59437.1 | 0 | 52 | 581 | 1 | 529 | cell wall invertase [Saccharum hybrid cultivar Pindar] |
| GenBank | ABB77250.1 | 0 | 5 | 581 | 4 | 576 | cell wall invertase BObetaFRUCT2 [Bambusa oldhamii] |
| GenBank | ACL53374.1 | 0 | 27 | 581 | 30 | 590 | unknown [Zea mays] |
| RefSeq | NP_001105369.1 | 0 | 27 | 581 | 30 | 590 | cell wall invertase1 precursor [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2xqr_K | 0 | 53 | 573 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 53 | 573 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 53 | 573 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 53 | 573 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_C | 0 | 53 | 573 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
