Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00058932m |
Family | GH13 |
Protein Properties | Length: 614 Molecular Weight: 68798.3 Isoelectric Point: 5.1458 |
Chromosome | Chromosome/Scaffold: 019431 Start: 283 End: 7326 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 342 | 603 | 3.9e-30 |
LPRIKRLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPEDLKSLIDKAHELGLLVLMDIVHSHASNNTLDGLNGFDGTDMHYFHGGPRGHHWMWD SRLFNYGSWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVAFTGNYGEYFGFATDVDAVVYLMLVNDLIHGLYPEAVAIGEDVSGMPTFCIP VQDGGVGFDYRLHMAAPDKWIELLKQSDEYWKMGDIVHTLTNRRWLEKCVTYAESHDQALVG |
Full Sequence |
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Protein Sequence Length: 614 Download |
MASFAVSGAR LGVVRPGGGA ARSGGERRSG VDLPPVLFRR KDSFSRTVLS CAGAPGKVLV 60 PGGGNDDLLS SAEPAVDNPV QSEQLEVPDE AELVVEESLA VEVEEKTEPS QVIEGVSETE 120 TDVLAVASKA PLMEEKPRVV PPPGDGQRIY QIDPMLEGFR NHLDYRYSEY KRMRAAIDQH 180 EGGLDAFSRG YEKLGFTRSA EGITYREWAP GAHSAALVGD FNNWNPNADT MTRNEYGTWE 240 IFLPNNADGS PAIPHGSRVK IRMDTPSGVK DSIPAWIKFS VQAPGEIPYN GIYYDPPEEE 300 KYVFKHPQPK RPKSLRIYES HVGMSSPEPK INTYANFRDE VLPRIKRLGY NAVQIMAIQE 360 HSYYASFGYH VTNFFAPSSR FGTPEDLKSL IDKAHELGLL VLMDIVHSHA SNNTLDGLNG 420 FDGTDMHYFH GGPRGHHWMW DSRLFNYGSW EVLRFLLSNA RWWLEEYKFD GFRFDGVTSM 480 MYTHHGLQVA FTGNYGEYFG FATDVDAVVY LMLVNDLIHG LYPEAVAIGE DVSGMPTFCI 540 PVQDGGVGFD YRLHMAAPDK WIELLKQSDE YWKMGDIVHT LTNRRWLEKC VTYAESHDQA 600 LVGDKTIAFW LMDK |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 1.0e-9 | 159 | 243 | 91 | + alpha-amylase | ||
PLN03244 | PLN03244 | 3.0e-94 | 251 | 608 | 361 | + alpha-amylase; Provisional | ||
PLN02960 | PLN02960 | 7.0e-132 | 251 | 608 | 361 | + alpha-amylase | ||
PLN02447 | PLN02447 | 0 | 106 | 614 | 516 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 298 | 614 | 318 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAB67316.1 | 0 | 32 | 614 | 1 | 597 | starch branching enzyme IIa [Zea mays] |
DDBJ | BAA82828.1 | 0 | 1 | 614 | 1 | 627 | starch branching enzyme rbe4 [Oryza sativa] |
EMBL | CAX51366.1 | 0 | 1 | 614 | 1 | 608 | starch branching enzyme [Hordeum vulgare subsp. vulgare] |
GenBank | EEE60949.1 | 0 | 1 | 614 | 1 | 627 | hypothetical protein OsJ_14706 [Oryza sativa Japonica Group] |
RefSeq | XP_002447772.1 | 0 | 1 | 614 | 1 | 610 | hypothetical protein SORBIDRAFT_06g015360 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 149 | 614 | 13 | 484 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 149 | 614 | 13 | 484 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 149 | 614 | 13 | 484 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 149 | 614 | 13 | 484 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 1m7x_D | 9.99967e-42 | 189 | 607 | 9 | 424 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 472 | 143 | 614 | 0 |
HO777638 | 416 | 199 | 614 | 0 |
HO458123 | 301 | 137 | 430 | 0 |
HO458123 | 183 | 432 | 614 | 0 |
HO777638 | 47 | 152 | 198 | 0.00000001 |
Sequence Alignments (This image is cropped. Click for full image.) |
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