Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00060573m |
Family | GH32 |
Protein Properties | Length: 248 Molecular Weight: 26599 Isoelectric Point: 6.5116 |
Chromosome | Chromosome/Scaffold: 0155972 Start: 1544 End: 2711 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 127 | 248 | 0 |
HFQPPKNWMNDPNGPLYHKGWYHLFYQWNPDSAVWGNITWGHAVSRDLVHWLHLPLAMVPDHWYDANGVWSGSATRLPDGRIVMLYTGSTTESVQVQNLA EPADPSDPLLRRWVKSAANPIL |
Full Sequence |
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Protein Sequence Length: 248 Download |
MITPVADPTM MDGDDARASL LPETDPRRRG PGGGGQKQPS SSTVLPAVVS AALLLVLAAV 60 AILASQHADD GQGGVAAAGR VVEVAASRGA AEGVSEKSTA PLLGAGGALR DYAWTNAMLA 120 WQRTAFHFQP PKNWMNDPNG PLYHKGWYHL FYQWNPDSAV WGNITWGHAV SRDLVHWLHL 180 PLAMVPDHWY DANGVWSGSA TRLPDGRIVM LYTGSTTESV QVQNLAEPAD PSDPLLRRWV 240 KSAANPIL |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
TIGR01322 | scrB_fam | 9.0e-33 | 123 | 214 | 92 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
COG1621 | SacC | 7.0e-41 | 123 | 248 | 130 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
cd08996 | GH32_B_Fructosidase | 9.0e-50 | 133 | 248 | 119 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
smart00640 | Glyco_32 | 3.0e-68 | 127 | 248 | 124 | + Glycosyl hydrolases family 32. |
pfam00251 | Glyco_hydro_32N | 5.0e-70 | 127 | 248 | 122 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAD41525.3 | 0 | 10 | 248 | 1 | 239 | OSJNBb0020O11.6 [Oryza sativa (japonica cultivar-group)] |
EMBL | CAH67112.1 | 0 | 10 | 248 | 1 | 239 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
GenBank | EEC77707.1 | 0 | 10 | 248 | 8 | 246 | hypothetical protein OsI_16783 [Oryza sativa Indica Group] |
Swiss-Prot | P49175 | 0 | 1 | 248 | 1 | 251 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
RefSeq | XP_002446857.1 | 0 | 1 | 248 | 1 | 263 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 112 | 248 | 8 | 144 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugh_A | 0 | 112 | 248 | 8 | 144 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_B | 0 | 112 | 248 | 8 | 144 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_A | 0 | 112 | 248 | 8 | 144 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugf_B | 0 | 112 | 248 | 8 | 144 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |