Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00063884m |
Family | GT20 |
Protein Properties | Length: 534 Molecular Weight: 58161.7 Isoelectric Point: 8.2251 |
Chromosome | Chromosome/Scaffold: 0221145 Start: 443 End: 2044 |
Description | trehalose-phosphatase/synthase 9 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 30 | 510 | 0 |
ARRIVVAHRLPLRASPDSDSPFGFAFSLDPDALSLQLSRGLPAPVTYIGTLPASAESKIVPSDELDGYLLEHFSCLPVHLDGDHHAEFYHGFCKHYLWPL LHYMLPFTPADVVGSLRFDAASYRAFIAANRQFAGRVIQVISSDAGDLVIVHDYHLWALPTFLRRKCPRAGVGFFLHSPFPSAEIVRSVPVRNELLGGLL NADLVGFHTLDYAHQFLSCCSRLLGISSNTSLNGHTNINYHGRTVLVKIFSVGVDMGQLRAALASPEAAAKAKEIAEAYRGRVIMVGDDDVDLFKGVRLK LLALEKMLEAHRDMARQVVLVQINNPARSRGRDVDAVRVETRRIEQRINKRFGSELIVVTIDGPVPMSEKVAYYAAADCCVVSPVRDGLNRIPYFYTVCR EEAPGARKSGAVVVSEFAGCSASLGGAIRVNPRNPEALADAMRAAVTMGAEEKRARHRTNYSYLSAHDVATWARSFDEALR |
Full Sequence |
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Protein Sequence Length: 534 Download |
MQGSSSSSCV HPSSAVAPAP PVPGADAPPA RRIVVAHRLP LRASPDSDSP FGFAFSLDPD 60 ALSLQLSRGL PAPVTYIGTL PASAESKIVP SDELDGYLLE HFSCLPVHLD GDHHAEFYHG 120 FCKHYLWPLL HYMLPFTPAD VVGSLRFDAA SYRAFIAANR QFAGRVIQVI SSDAGDLVIV 180 HDYHLWALPT FLRRKCPRAG VGFFLHSPFP SAEIVRSVPV RNELLGGLLN ADLVGFHTLD 240 YAHQFLSCCS RLLGISSNTS LNGHTNINYH GRTVLVKIFS VGVDMGQLRA ALASPEAAAK 300 AKEIAEAYRG RVIMVGDDDV DLFKGVRLKL LALEKMLEAH RDMARQVVLV QINNPARSRG 360 RDVDAVRVET RRIEQRINKR FGSELIVVTI DGPVPMSEKV AYYAAADCCV VSPVRDGLNR 420 IPYFYTVCRE EAPGARKSGA VVVSEFAGCS ASLGGAIRVN PRNPEALADA MRAAVTMGAE 480 EKRARHRTNY SYLSAHDVAT WARSFDEALR LACRDHAAMM FVGLGFGMSY RAVA 540 |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
COG0380 | OtsA | 9.0e-99 | 31 | 512 | 493 | + Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism] |
TIGR02400 | trehalose_OtsA | 6.0e-99 | 31 | 510 | 491 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. |
cd03788 | GT1_TPS | 1.0e-132 | 31 | 510 | 485 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. |
pfam00982 | Glyco_transf_20 | 9.0e-143 | 31 | 509 | 485 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. |
PLN02205 | PLN02205 | 1.0e-155 | 31 | 534 | 521 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAD09488.1 | 0 | 30 | 534 | 23 | 534 | putative trehalose-6-phosphate synthase [Oryza sativa Japonica Group] |
DDBJ | BAD33622.1 | 0 | 31 | 534 | 118 | 631 | putative SL-TPS/P [Oryza sativa Japonica Group] |
DDBJ | BAH94577.1 | 0 | 31 | 534 | 48 | 561 | Os09g0427800 [Oryza sativa Japonica Group] |
GenBank | EAZ44803.1 | 0 | 31 | 534 | 48 | 561 | hypothetical protein OsJ_29437 [Oryza sativa Japonica Group] |
RefSeq | XP_002453209.1 | 0 | 24 | 534 | 17 | 532 | hypothetical protein SORBIDRAFT_04g001670 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1uqu_B | 1e-37 | 116 | 510 | 75 | 452 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqu_A | 1e-37 | 116 | 510 | 75 | 452 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqt_B | 1e-37 | 116 | 510 | 75 | 452 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqt_A | 1e-37 | 116 | 510 | 75 | 452 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1gz5_D | 1e-37 | 116 | 510 | 74 | 451 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 501 | 53 | 534 | 0 |
HO779327 | 473 | 74 | 534 | 0 |
DT653950 | 297 | 49 | 343 | 0 |
DT944686 | 272 | 72 | 343 | 0 |
EE159861 | 272 | 72 | 343 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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