Basic Information | |
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Species | Phaseolus vulgaris |
Cazyme ID | Phvul.001G036600.1 |
Family | GH32 |
Protein Properties | Length: 557 Molecular Weight: 63633.5 Isoelectric Point: 5.1732 |
Chromosome | Chromosome/Scaffold: 01 Start: 3524214 End: 3527348 |
Description | 6-&1-fructan exohydrolase |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 28 | 347 | 0 |
HFQPPQNWMNDPNGPMYYKGVYHFFYQFNPYAPTFGRHMVWGHSVSYDLINWIHLNHAIEPSESYDINGCYSGSITILPGEKPIIMYTGSDSNKHQVQNL AVPKNLSDTFLREWEKHPQNPILIPPSGVEVDGFRDPTTAWQGSDGKWRMIIGAKNGDEGKALLYHSEDFVNWKPFPNPMYASENTGMFECPDFFPVYIS GTKNGVDTSVQNSSVKHVLKTSYQNKQLEYYFVGDYFPDQEKFVPDDDLGGPNLTLILDHGSFYASKSFFDYAKNRRILWGWSQECDTTQDDNEKGWAGL QSIPRQVWLDASGKMLMQWP |
Full Sequence |
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Protein Sequence Length: 557 Download |
METKASLDSI NSNKYMVPQK QPYRTWYHFQ PPQNWMNDPN GPMYYKGVYH FFYQFNPYAP 60 TFGRHMVWGH SVSYDLINWI HLNHAIEPSE SYDINGCYSG SITILPGEKP IIMYTGSDSN 120 KHQVQNLAVP KNLSDTFLRE WEKHPQNPIL IPPSGVEVDG FRDPTTAWQG SDGKWRMIIG 180 AKNGDEGKAL LYHSEDFVNW KPFPNPMYAS ENTGMFECPD FFPVYISGTK NGVDTSVQNS 240 SVKHVLKTSY QNKQLEYYFV GDYFPDQEKF VPDDDLGGPN LTLILDHGSF YASKSFFDYA 300 KNRRILWGWS QECDTTQDDN EKGWAGLQSI PRQVWLDASG KMLMQWPIEE VEKLRDNQIS 360 IKGEKLAGGS ILEVSGITAS QADVEVLFEL PELENAEFID ESEVDPHLLC SEEYASRSGI 420 IGPFGLLALA SEDQTEHTAI FFRIYRAPNR FVCLMCSDHR RSSLRQNLEK SSYRTIFDID 480 PKQKTISLRC LIDRSIIESF GEKGRICITS RVYPSLAIDK DAHLYVFNNG RQSVVISELN 540 AWSMKQAEFG QEQSIK* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 3.0e-59 | 19 | 515 | 520 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-78 | 19 | 515 | 515 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-97 | 34 | 350 | 331 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-141 | 28 | 347 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-163 | 28 | 504 | 485 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAC37922.1 | 0 | 2 | 556 | 22 | 578 | fructan 1-exohydrolase IIa [Cichorium intybus] |
EMBL | CAC37923.1 | 0 | 20 | 556 | 42 | 578 | fructan 1-exohydrolase IIb [Cichorium intybus] |
EMBL | CAD49079.1 | 0 | 6 | 555 | 28 | 574 | fructan 1-exohydrolase [Campanula rapunculoides] |
RefSeq | XP_002278918.1 | 0 | 1 | 549 | 20 | 559 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
RefSeq | XP_002309497.1 | 0 | 16 | 554 | 39 | 571 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 20 | 556 | 4 | 540 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
PDB | 2ade_A | 0 | 20 | 556 | 4 | 540 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
PDB | 2add_A | 0 | 20 | 556 | 4 | 540 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
PDB | 1st8_A | 0 | 20 | 556 | 4 | 540 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 20 | 556 | 4 | 540 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CT842376 | 531 | 24 | 546 | 0 |
FE693031 | 280 | 267 | 546 | 0 |
BE659875 | 231 | 324 | 554 | 0 |
GR835427 | 219 | 316 | 534 | 0 |
EE591202 | 425 | 147 | 555 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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