Basic Information | |
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Species | Phaseolus vulgaris |
Cazyme ID | Phvul.002G243600.1 |
Family | GT20 |
Protein Properties | Length: 924 Molecular Weight: 104733 Isoelectric Point: 7.1067 |
Chromosome | Chromosome/Scaffold: 02 Start: 41045416 End: 41059439 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 85 | 554 | 0 |
PNKQRLLVVANRLPVSAVRDGVESYQLEISVGGLVSALLGVKEFDTRWIGWAGVNVPDDVGRRALTKALAEMRCIPVFLDEEIVNQYYNGYCNNILWPLF HYLGLPQEDRLATTRTFQSQFDAYKEANQIFADVVHQHYEEGDVVWCHDYHLMFLPKCLKERNDKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADL VGFHTYDYARHFVSACTRILGLEGTPDGVEDRGKLTRVAAFPIGIDSERFIQALELPEVQEHMKELKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLE ENSHWRNKVVLLQIAVPTRKDVPEYQKLASQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHELCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVL ILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALDMPADEREKRHQFNFKHVKTHTSQEWAATFVSELN |
Full Sequence |
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Protein Sequence Length: 924 Download |
MFQSKRTPAN TYNYVPLTPR SRLERLLRER ELRKSFKNTL PTDEARDGNK EGEQCCSDSS 60 ISENEISGVH KEEEATETNK QDERPNKQRL LVVANRLPVS AVRDGVESYQ LEISVGGLVS 120 ALLGVKEFDT RWIGWAGVNV PDDVGRRALT KALAEMRCIP VFLDEEIVNQ YYNGYCNNIL 180 WPLFHYLGLP QEDRLATTRT FQSQFDAYKE ANQIFADVVH QHYEEGDVVW CHDYHLMFLP 240 KCLKERNDKM KVGWFLHTPF PSSEIHRTLP SRSELLRSVL AADLVGFHTY DYARHFVSAC 300 TRILGLEGTP DGVEDRGKLT RVAAFPIGID SERFIQALEL PEVQEHMKEL KERFAGRKVM 360 LGVDRLDMIK GIPQKILAFE KFLEENSHWR NKVVLLQIAV PTRKDVPEYQ KLASQVHEIV 420 GRINGRFGTL TAVPIHHLDR SLDFHELCAL YAVTDVALVT SLRDGMNLVS YEFVACQASK 480 KGVLILSEFA GAAQSLGAGA ILVNPWNITE VAASIGYALD MPADEREKRH QFNFKHVKTH 540 TSQEWAATFV SELNDTIVEA QLRTRQDPPL LPNKVAVDCY SKSNNRLIIL GFNATLTEPV 600 DSLGRAGQIR ELELKLHPNL KETLKKLSDD PQTTIVLLSG SNRAVLDKNF SEFNMWLAAE 660 NGMFLRLTSS EWMTTMPENL CMDWVDSVKH VFEYFTERTP RSHFELRETS IVWNYKYADV 720 EFGRIQARDL LQHLWTGPIS NASLDVVQGG RSVEVRTVGV SKGAAIDRIL GEIVHNKGMK 780 APIDYVLCIG HFLAKDEDVY KFFEPELPCE SPPVPRAMLC KSNSYRPSSV QKLTANRAGI 840 KTAHYKKQRS LSNIEKREID QSRPTYRDKI SQHEGSSVLD LKGDNYFSCV VGRKRSSARY 900 LLKTSDDVVN LLSDLAKHSS PTT* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 8 | 922 | 934 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 82 | 923 | 842 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 89 | 555 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 88 | 555 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
PRK14501 | PRK14501 | 0 | 89 | 808 | 727 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | NP_177979.1 | 0 | 8 | 920 | 2 | 940 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002285632.1 | 0 | 8 | 920 | 2 | 925 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002305707.1 | 0 | 27 | 921 | 1 | 898 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002522296.1 | 0 | 8 | 920 | 2 | 913 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
RefSeq | XP_002531237.1 | 0 | 8 | 922 | 2 | 926 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 89 | 565 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DW069910 | 288 | 175 | 462 | 0 |
ES813375 | 375 | 450 | 816 | 0 |
CV711307 | 294 | 86 | 379 | 0 |
CX546763 | 293 | 86 | 378 | 0 |
CB651133 | 280 | 116 | 395 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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