| Basic Information | |
|---|---|
| Species | Phaseolus vulgaris |
| Cazyme ID | Phvul.003G217900.1 |
| Family | GH32 |
| Protein Properties | Length: 652 Molecular Weight: 72629.7 Isoelectric Point: 6.4672 |
| Chromosome | Chromosome/Scaffold: 03 Start: 43438908 End: 43442998 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 120 | 440 | 0 |
| HFQPEKNWMNDPNGPMYYKGWYHFFYQYNPNGAVWGDIVWGHAVSRDMIHWLHLPLAMVADQWYDKQGVWTGSATILPNGEIIMLYTGSTNESVQVQNLA YPADPSDPLLVDWIKHPGNPVLVPPPGIGAKDFRDPTTAWLTSEGKWRITIGSKLNKTGIALVYDTDDFKTYELKNGHLRAVPGTGMWECVDFFPVSKKN ENGLDTSLSINGAEVKYVMKVSLDDDRHDYYTIGTYDENKVLFTPDDVKNDVGVGLRYDYGIFYASKTFYDQNMDRRILWGWIGESDSEYADVTKGWASV QSIPRTVRLDKKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 652 Download |
| MEHHKPLLPT SSHAAPNPRT RKDLLLLLCA LLFLSSLVAF GRNRASNVPH DHVSSSASNH 60 QQEHQSPTSL PSSKWHAVSR GVSSGVSEKS SSMLFSGEGG ASEAFPWDNS MLSWQRTSFH 120 FQPEKNWMND PNGPMYYKGW YHFFYQYNPN GAVWGDIVWG HAVSRDMIHW LHLPLAMVAD 180 QWYDKQGVWT GSATILPNGE IIMLYTGSTN ESVQVQNLAY PADPSDPLLV DWIKHPGNPV 240 LVPPPGIGAK DFRDPTTAWL TSEGKWRITI GSKLNKTGIA LVYDTDDFKT YELKNGHLRA 300 VPGTGMWECV DFFPVSKKNE NGLDTSLSIN GAEVKYVMKV SLDDDRHDYY TIGTYDENKV 360 LFTPDDVKND VGVGLRYDYG IFYASKTFYD QNMDRRILWG WIGESDSEYA DVTKGWASVQ 420 SIPRTVRLDK KTGSNLLQWP VAEVESLRLR SDEFKSLKAK PGSVVSLDIE TATQLDVVAE 480 FVIDAESLQK TAQSNEEFTC STSGGAAQRG ALGPFGLLVL ADEGLSEYTP VYFYVIKGRN 540 GNLKTSFCSD QSRSSQANDV RKQIFGNIVP VLEGEKFSLR MLVDHSIVES FAQGGRTCVT 600 SRVYPTKAIY GAARLFLFNN ATEATVTASL KIWQMNSAFI RPFPFNPDQK N* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 4.0e-61 | 116 | 605 | 509 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-75 | 116 | 634 | 533 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-100 | 126 | 443 | 330 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-154 | 120 | 440 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 120 | 595 | 486 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM52062.1 | 0 | 72 | 644 | 68 | 638 | vacuolar acid invertase PsI-1 [Pisum sativum] |
| GenBank | ACT21538.1 | 0 | 71 | 651 | 1 | 579 | acid invertase [Vigna radiata] |
| Swiss-Prot | O24509 | 0 | 1 | 651 | 1 | 651 | INVA_PHAVU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Flags: Precursor |
| Swiss-Prot | P29001 | 0 | 1 | 651 | 1 | 649 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| Swiss-Prot | Q43857 | 0 | 78 | 650 | 69 | 638 | INVA_VICFA RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 105 | 643 | 8 | 544 | A Chain A, The Structure Of Soybean Peroxidase |
| PDB | 3ugh_A | 0 | 105 | 643 | 8 | 544 | A Chain A, The Structure Of Soybean Peroxidase |
| PDB | 3ugg_B | 0 | 105 | 643 | 8 | 544 | A Chain A, The Structure Of Soybean Peroxidase |
| PDB | 3ugg_A | 0 | 105 | 643 | 8 | 544 | A Chain A, The Structure Of Soybean Peroxidase |
| PDB | 3ugf_B | 0 | 105 | 643 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO797746 | 300 | 353 | 652 | 0 |
| FD794501 | 256 | 138 | 393 | 0 |
| FG890042 | 244 | 101 | 344 | 0 |
| FD795909 | 240 | 299 | 538 | 0 |
| DY919082 | 298 | 143 | 440 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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