Basic Information | |
---|---|
Species | Phaseolus vulgaris |
Cazyme ID | Phvul.005G008200.1 |
Family | GH32 |
Protein Properties | Length: 634 Molecular Weight: 71331.1 Isoelectric Point: 6.4222 |
Chromosome | Chromosome/Scaffold: 05 Start: 708577 End: 714115 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
---|
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH32 | 114 | 432 | 0 |
HFQPQKNWMNDPDGPLFHMGWYHLFYQYNPDSAVWGNITWGHAVSRDMIHWLYLPIAMVPNDWYDINGVWTGSATILPGGKIIMLYTGDTNHYVQVQNLA YPANLSDPLLLHWVKYPGNPILLPPPGIGSKDFRDPTTGWIGSDGKWRITIGSKKGKTGLSLVYKTTDFINFEPNDHYLHAVPGTGMWECVDFYPVSING SKGLDSSVNGPYVKHVLKASLDDKRVDSYALGTYFVENDTWVPDDPFMDVGIGLLLDYGRYYASKSFYDEEKERRILWGWVNETDIESDDLRKGWASLQT IPRTVLFDNKTRTNLLLWP |
Full Sequence |
---|
Protein Sequence Length: 634 Download |
MEANLSNPHD LHTPLMNHPP RLNKNGHKPS KGILVILCSI VFLMSLFALI MIKSQNHLEK 60 MEIGNTETTP FDPNISPARG VAQGVSPKSI PTLFHKVSYN WTNAMFSWQR TAFHFQPQKN 120 WMNDPDGPLF HMGWYHLFYQ YNPDSAVWGN ITWGHAVSRD MIHWLYLPIA MVPNDWYDIN 180 GVWTGSATIL PGGKIIMLYT GDTNHYVQVQ NLAYPANLSD PLLLHWVKYP GNPILLPPPG 240 IGSKDFRDPT TGWIGSDGKW RITIGSKKGK TGLSLVYKTT DFINFEPNDH YLHAVPGTGM 300 WECVDFYPVS INGSKGLDSS VNGPYVKHVL KASLDDKRVD SYALGTYFVE NDTWVPDDPF 360 MDVGIGLLLD YGRYYASKSF YDEEKERRIL WGWVNETDIE SDDLRKGWAS LQTIPRTVLF 420 DNKTRTNLLL WPVEEVESLR LSSDEFEGMV VKPGSVVPLN ISLATQLDIF AEFEIEWLNS 480 KSNGKNNMGC GKGGATDRSA FGPFGLLAIA DDSLSEQTPI YFRLSNTTTF FCVDETRSSK 540 ASDVSKAIYG SNIPILSDEK LSMRVLVDHS IIESFAQGGR RVITSRVYPT EAIYGAARLF 600 VFNNATDINI KISLKIWQLN SAFLRPFPFD QSH* |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-46 | 110 | 589 | 496 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-61 | 110 | 591 | 493 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-81 | 120 | 435 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 7.0e-139 | 114 | 432 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-170 | 114 | 579 | 476 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 35 | 627 | 36 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF34363.1 | 0 | 79 | 631 | 85 | 641 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35858.1 | 0 | 55 | 631 | 68 | 644 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 55 | 631 | 68 | 644 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 9 | 632 | 11 | 639 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 95 | 627 | 4 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugh_A | 0 | 95 | 627 | 4 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugg_B | 0 | 95 | 627 | 4 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugg_A | 0 | 95 | 627 | 4 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugf_B | 0 | 95 | 627 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |