| Basic Information | |
|---|---|
| Species | Phaseolus vulgaris |
| Cazyme ID | Phvul.009G261000.1 |
| Family | GT68 |
| Protein Properties | Length: 530 Molecular Weight: 60494.5 Isoelectric Point: 8.3687 |
| Chromosome | Chromosome/Scaffold: 09 Start: 37323053 End: 37325604 |
| Description | O-fucosyltransferase family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GT68 | 190 | 504 | 0 |
| NHLMCLQKHMFFAALLNRVLVIPSSKVDYEYERVLDVEHMNRCAGGKVKAVISFQEFSSMKKNGMHEGKFLCYFSLPRPCYLDDEHLRKLKSLGLLSTNK PAAAWDEDARKPRKRSVEEVVSRFSREEKEDVLVIGDVLYADVEEEWVTQAGGPLAHRCKTLIQPNRLIFLTAQRFIQTFLGRNFLALHFRRHDFLNFCN GKKPSCFYPIPQAAACILRVVERANAPLIYLSTDAAESEIRLLQSLLVLEGRHVPLVKRPPRNSAEKWDALLYRHHIEGDPQVEAMLDKTICAMSSVFIG ASGSTFTQDIRRLRK | |||
| Full Sequence |
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| Protein Sequence Length: 530 Download |
| MERDSSSDEE EELIHQKQGN APRSPSSFHF QSLTSRITSN FNFNFKFPFQ KRYLFAILPL 60 FAILLYYLLP DSHSLFSLSS VNLNLRHDQP EESQLRALYL LRQQQSGLLH ALNSTSQTHI 120 LKSLLSNQIS LNSQIQHLLL SPYTTATSLH FRNAACGTVD QKLLERRTME WKPKANKFLV 180 AVCVSGQMSN HLMCLQKHMF FAALLNRVLV IPSSKVDYEY ERVLDVEHMN RCAGGKVKAV 240 ISFQEFSSMK KNGMHEGKFL CYFSLPRPCY LDDEHLRKLK SLGLLSTNKP AAAWDEDARK 300 PRKRSVEEVV SRFSREEKED VLVIGDVLYA DVEEEWVTQA GGPLAHRCKT LIQPNRLIFL 360 TAQRFIQTFL GRNFLALHFR RHDFLNFCNG KKPSCFYPIP QAAACILRVV ERANAPLIYL 420 STDAAESEIR LLQSLLVLEG RHVPLVKRPP RNSAEKWDAL LYRHHIEGDP QVEAMLDKTI 480 CAMSSVFIGA SGSTFTQDIR RLRKDWGSAS LCDEYLCQGE EPNVVAEIE* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam10250 | O-FucT | 0.0008 | 201 | 503 | 329 | + GDP-fucose protein O-fucosyltransferase. This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. | ||
| cd11302 | O-FucT-1 | 0.0003 | 352 | 504 | 178 | + GDP-fucose protein O-fucosyltransferase 1. The protein O-fucosyltransferase 1 (Ofut1 or O-FucT-1) adds O-fucose to EGF (epidermal growth factor-like) repeats. The O-fucsosylation of the Notch receptor signaling protein is dependent on this enzyme, which requires GDP-fucose as a substrate. O-fucose residues added to the target of O-FucT-1 may be further elongated by other glycosyltransferases. On top of O-fucosylation, O-FucT-1 may have other functions such as the regulation of the Notch receptor exit from the ER. Six highly conserved cysteines are present in O-FucT-1, which is a soluble ER protein, as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The membrane-bound pre-protein is released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. O-FucT-1 is similar to family 1 glycosyltransferases (GT1). | ||
| cd11296 | O-FucT_like | 0.0002 | 178 | 221 | 44 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| cd11298 | O-FucT-2 | 6.0e-10 | 370 | 500 | 133 | + GDP-fucose protein O-fucosyltransferase 2. O-FucT-2 adds O-fucose to thrombospondin type 1 repeats (TSRs), and appears conserved in bilateria. The O-fucosylation of TSRs appears to play a role in regulating secretion of metalloproteases of the ADAMTS superfamily. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| cd11296 | O-FucT_like | 6.0e-20 | 343 | 503 | 176 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM66093.1 | 0 | 1 | 529 | 1 | 566 | unknown [Arabidopsis thaliana] |
| RefSeq | NP_173170.2 | 0 | 19 | 529 | 42 | 564 | unknown protein [Arabidopsis thaliana] |
| RefSeq | NP_199853.1 | 0 | 1 | 529 | 1 | 566 | unknown protein [Arabidopsis thaliana] |
| RefSeq | XP_002264087.1 | 0 | 1 | 529 | 1 | 559 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002303337.1 | 0 | 92 | 529 | 72 | 527 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4ap5_B | 0.002 | 370 | 504 | 263 | 377 | A Chain A, Crystal Structure Of Human Pofut2 |
| PDB | 4ap5_A | 0.002 | 370 | 504 | 263 | 377 | A Chain A, Crystal Structure Of Human Pofut2 |
| PDB | 4ap6_D | 0.002 | 370 | 504 | 277 | 391 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_C | 0.002 | 370 | 504 | 277 | 391 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_B | 0.002 | 370 | 504 | 277 | 391 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
