Basic Information | |
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Species | Populus trichocarpa |
Cazyme ID | Potri.001G139500.3 |
Family | GT20 |
Protein Properties | Length: 681 Molecular Weight: 77091.5 Isoelectric Point: 6.8182 |
Chromosome | Chromosome/Scaffold: 01 Start: 11206601 End: 11210120 |
Description | trehalose phosphatase/synthase 5 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 57 | 547 | 0 |
SLGRMIIVGNQLPLRAHRSPDSSGGWCFSWDEDSLLLQLKDGLGEGVEVIYVGSLKEEIEPSEQDDVAQTLLETFKCVPAFIPPDLFTKFYHGFCKQHLW PLFHYMLPLSPDLGGRFDRSLWQAYVSVNKIFADKVKEVISPEDDYVWVHDYHLMVLPTFLRKIFNRVKLGFFLHSPFPSSEIYRTLPVRDELLRALLNS DLIGFHTFDYARHFLSCCSRMLGLSYQSKRGYIGLEYFGRTVSIKILPVGIHIGQLQSVLNLPETESKVAELHDQFRGQAVMLGVDDMDIFKGISLKLLA MEELLTQHPNKRGEVVLVQIANPARGRGREVQEVQSETKAAVRRINEAFGSPGYTPVVLIDRPLQFYERIAYYAIAECCLVTAVRDGMNLIPYEYIICRQ GNEKLDETLGRDPSAPRKSMLVLSEFIGCSPSLSGAIRVNPWNIDAVAEAMNSALVVPEPEKQMRHEKHHRYVSTHDVAYWARSFLQDLER |
Full Sequence |
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Protein Sequence Length: 681 Download |
MVSRSYSNLL DLASGDAPIP SFGRERKRFP RVATVAGILT DLDDENNVGS DSPSSVSLGR 60 MIIVGNQLPL RAHRSPDSSG GWCFSWDEDS LLLQLKDGLG EGVEVIYVGS LKEEIEPSEQ 120 DDVAQTLLET FKCVPAFIPP DLFTKFYHGF CKQHLWPLFH YMLPLSPDLG GRFDRSLWQA 180 YVSVNKIFAD KVKEVISPED DYVWVHDYHL MVLPTFLRKI FNRVKLGFFL HSPFPSSEIY 240 RTLPVRDELL RALLNSDLIG FHTFDYARHF LSCCSRMLGL SYQSKRGYIG LEYFGRTVSI 300 KILPVGIHIG QLQSVLNLPE TESKVAELHD QFRGQAVMLG VDDMDIFKGI SLKLLAMEEL 360 LTQHPNKRGE VVLVQIANPA RGRGREVQEV QSETKAAVRR INEAFGSPGY TPVVLIDRPL 420 QFYERIAYYA IAECCLVTAV RDGMNLIPYE YIICRQGNEK LDETLGRDPS APRKSMLVLS 480 EFIGCSPSLS GAIRVNPWNI DAVAEAMNSA LVVPEPEKQM RHEKHHRYVS THDVAYWARS 540 FLQDLERACR DHVKRRCWGF GFGLGFRVIA LDPNFRKISV EHIVSAYKRT KNRVILLDYD 600 GTMTLPSSTR TPNMETVGVL NSLCTDPKNV VFLVSGRDRE TLTEWFSSCE KLGIAAEHGY 660 FVRYKLPFLT PFQFDLDLPT * |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR02400 | trehalose_OtsA | 5.0e-152 | 60 | 547 | 491 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
PRK14501 | PRK14501 | 1.0e-152 | 59 | 663 | 611 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
PLN02205 | PLN02205 | 0 | 1 | 665 | 672 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
pfam00982 | Glyco_transf_20 | 0 | 59 | 547 | 493 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
cd03788 | GT1_TPS | 0 | 60 | 546 | 487 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAN61971.1 | 0 | 1 | 663 | 1 | 664 | hypothetical protein [Vitis vinifera] |
RefSeq | NP_567538.1 | 0 | 1 | 663 | 1 | 663 | ATTPS5; protein binding / transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
RefSeq | XP_002277503.1 | 0 | 1 | 663 | 1 | 640 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002304347.1 | 0 | 1 | 663 | 1 | 662 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002326738.1 | 0 | 1 | 662 | 1 | 662 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1gz5_D | 0 | 145 | 547 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_C | 0 | 145 | 547 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_B | 0 | 145 | 547 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_A | 0 | 145 | 547 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1uqu_B | 0 | 145 | 547 | 75 | 453 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 601 | 70 | 665 | 0 |
HO779327 | 510 | 103 | 612 | 0 |
DT564774 | 294 | 113 | 406 | 0 |
HO779327 | 57 | 609 | 665 | 0.0000000000003 |
HO777775 | 80 | 1 | 77 | 0.00000009 |
Sequence Alignments (This image is cropped. Click for full image.) |
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