| Basic Information | |
|---|---|
| Species | Populus trichocarpa |
| Cazyme ID | Potri.004G123600.5 |
| Family | GT35 |
| Protein Properties | Length: 989 Molecular Weight: 112099 Isoelectric Point: 5.5106 |
| Chromosome | Chromosome/Scaffold: 04 Start: 11895488 End: 11905810 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT35 | 175 | 983 | 0 |
| ALSKLGHSLENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQQITKDGQEEVAEDWLEMGNPWEILRNDISYPIKFYGKVVSGS DGKKHWIGGEDIKAVAYDVPIPGYKTKTTINLRLWSTKAPSEDLDLYAFNAGDHTKAYEALSNAEKICHILYPGDDSLEGKILRLKQQYTLCSASLQDII SCFERRSGSNIDWEKFPEKVAVQMNDTHPTLCIPELMRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSLELMQKLLPRHVEIIELIDEELICT IVSEYGTEDSDLLEKKLKEMRILENVDLPSAFAELIVKPKQSSVETKRANDFEEETKRANDLEEETNLEEETKRANDFEEEMELVDEKDESKSKVTQKKE KIMAEPPPKPPKMVRMANLAVVGGHAVNGVAEIHSEIVKDEVFNAFYKLWPDKFQNKTNGVTPRRWIHFCNPGLSKIITDWIGMDDWVLNTEKLAELRKF SDNEDLQVQWKAAKRSNKMKVISFLKEKTGYSVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMTAAERKAKYVPRVCIFGGKAFSTYVQAKRIV KFITDVGATVNHDPEIGDLLKVVFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGARAHEIAG LRKERADGEFVPDPSFEEVKDFVKSGVFGPCNYDELIGSLEGNEGFGRADYFLVGKDFPSYIECQEEVDKAYHDQKTWTKMSIMNTAGSYKFSSDRTIHE YAREIWNIE | |||
| Full Sequence |
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| Protein Sequence Length: 989 Download |
| MATTSSHFSP TSHWCSNGSS ISRLVDFGSK WRRKQQLFSM NSRRVVKRRS VSVSIKNVSS 60 SEPKQKLKDD ALIEEEVPRI LNPSTPNASS IASSIKYHAE FTPLFSPERF ELPKAYYATA 120 QSVRDALIIN WNSTYESYER LNAKQAYYLS MEFLQGRALL NAIGNLELTG AYAEALSKLG 180 HSLENVACQE PDAALGNGGL GRLASCFLDS LATLNYPAWG YGLRYKYGLF KQQITKDGQE 240 EVAEDWLEMG NPWEILRNDI SYPIKFYGKV VSGSDGKKHW IGGEDIKAVA YDVPIPGYKT 300 KTTINLRLWS TKAPSEDLDL YAFNAGDHTK AYEALSNAEK ICHILYPGDD SLEGKILRLK 360 QQYTLCSASL QDIISCFERR SGSNIDWEKF PEKVAVQMND THPTLCIPEL MRILIDLKGL 420 SWKEAWNITQ RTVAYTNHTV LPEALEKWSL ELMQKLLPRH VEIIELIDEE LICTIVSEYG 480 TEDSDLLEKK LKEMRILENV DLPSAFAELI VKPKQSSVET KRANDFEEET KRANDLEEET 540 NLEEETKRAN DFEEEMELVD EKDESKSKVT QKKEKIMAEP PPKPPKMVRM ANLAVVGGHA 600 VNGVAEIHSE IVKDEVFNAF YKLWPDKFQN KTNGVTPRRW IHFCNPGLSK IITDWIGMDD 660 WVLNTEKLAE LRKFSDNEDL QVQWKAAKRS NKMKVISFLK EKTGYSVSPD AMFDIQVKRI 720 HEYKRQLLNI LGIVYRYKKM KEMTAAERKA KYVPRVCIFG GKAFSTYVQA KRIVKFITDV 780 GATVNHDPEI GDLLKVVFVP DYNVSVAELL IPASELSQHI STAGMEASGT SNMKFAMNGC 840 ILIGTLDGAN VEIREEVGED NFFLFGARAH EIAGLRKERA DGEFVPDPSF EEVKDFVKSG 900 VFGPCNYDEL IGSLEGNEGF GRADYFLVGK DFPSYIECQE EVDKAYHDQK TWTKMSIMNT 960 AGSYKFSSDR TIHEYAREIW NIEPVELP* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 2.0e-129 | 175 | 498 | 324 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 3.0e-176 | 92 | 460 | 373 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 584 | 982 | 404 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 586 | 984 | 404 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 586 | 982 | 402 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 54 | 988 | 5 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| EMBL | CBI22291.1 | 0 | 13 | 988 | 14 | 982 | unnamed protein product [Vitis vinifera] |
| Swiss-Prot | P53536 | 0 | 1 | 987 | 1 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 40 | 988 | 1 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 988 | 1 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 586 | 984 | 438 | 830 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1z8d_A | 0 | 87 | 498 | 24 | 433 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 2zb2_B | 0 | 586 | 987 | 440 | 835 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Glcose And 5- Chloro-N-[4-(1,2-Dihydroxyethyl)phenyl]-1h-Indole-2-Carboxamide |
| PDB | 2zb2_B | 0 | 87 | 498 | 26 | 435 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Glcose And 5- Chloro-N-[4-(1,2-Dihydroxyethyl)phenyl]-1h-Indole-2-Carboxamide |
| PDB | 2zb2_A | 0 | 586 | 987 | 440 | 835 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Glcose And 5- Chloro-N-[4-(1,2-Dihydroxyethyl)phenyl]-1h-Indole-2-Carboxamide |
| Metabolic Pathways | |||
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| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO778303 | 875 | 115 | 989 | 0 |
| HO797178 | 384 | 606 | 989 | 0 |
| HO620767 | 403 | 587 | 989 | 0 |
| HO613954 | 403 | 587 | 989 | 0 |
| HO778303 | 55 | 62 | 116 | 0.00000002 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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