y
Basic Information | |
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Species | Populus trichocarpa |
Cazyme ID | Potri.006G115100.6 |
Family | GH13 |
Protein Properties | Length: 558 Molecular Weight: 64538.8 Isoelectric Point: 5.6291 |
Chromosome | Chromosome/Scaffold: 06 Start: 9007009 End: 9020579 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 80 | 400 | 4.4e-29 |
LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNYFAPCSRCGTPDDLKSLIDRAHELGLLVLMDIVHSHASNNTLDGLNMFDGTDNHYFHSGSRGHHWMWD SRLFNYGSWEVLRFLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLQMTFTGNYNEYFGYATDIDAVVYLMVVNDMIHGLFPDAVSIGEDVSGMPTFCIP VQDGGVGFDYRLHMAIADKWIELLQKKDEDWRMGDIVHTLTNRRWLEKCVSYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPSTPLVDRGIALHKMIR LITMGLGGEGYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 558 Download |
MDTPSGIKDS IPAWIKFSVQ APGEIPYNGI YYDPPEEEKY IFKHPQPKRP ESLRIYEAHV 60 GMSSTEPLIN TYANFRDDVL PRIKKLGYNA VQIMAIQEHS YYASFGYHVT NYFAPCSRCG 120 TPDDLKSLID RAHELGLLVL MDIVHSHASN NTLDGLNMFD GTDNHYFHSG SRGHHWMWDS 180 RLFNYGSWEV LRFLLSNARW WLDEYKFDGF RFDGVTSMMY THHGLQMTFT GNYNEYFGYA 240 TDIDAVVYLM VVNDMIHGLF PDAVSIGEDV SGMPTFCIPV QDGGVGFDYR LHMAIADKWI 300 ELLQKKDEDW RMGDIVHTLT NRRWLEKCVS YAESHDQALV GDKTIAFWLM DKDMYDFMAL 360 DRPSTPLVDR GIALHKMIRL ITMGLGGEGY LNFMGNEFGH PEWIDFPRGD QRLPTGKIIP 420 GNNNSFDKCR RRFDLGDAEY LRYHGMQEFD RAMQHLEEIY GFMTSEHQYI SRKNEGDRVI 480 VFERGNLVFV FNFHWTNSYS DYRVGCLKPG KYKIVLDSDD PLFGGFKRLD KDAEYFSSEG 540 WYDDRPRSFL VYAPSGG* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG0296 | GlgB | 2.0e-94 | 1 | 554 | 572 | + 1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism] | ||
PLN03244 | PLN03244 | 1.0e-133 | 2 | 525 | 530 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 1 | 555 | 559 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 36 | 451 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 3 | 532 | 533 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05322.1 | 0 | 1 | 555 | 261 | 815 | starch branching enzyme II [Populus trichocarpa] |
EMBL | CAA56319.1 | 0 | 1 | 555 | 282 | 836 | starch branching enzyme I [Pisum sativum] |
RefSeq | XP_002277213.1 | 0 | 1 | 554 | 497 | 1050 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002326414.1 | 0 | 1 | 555 | 158 | 712 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002534111.1 | 0 | 1 | 555 | 274 | 828 | starch branching enzyme II, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 9 | 554 | 135 | 683 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 9 | 554 | 135 | 683 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 9 | 554 | 135 | 683 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 9 | 554 | 135 | 683 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3k1d_A | 8e-35 | 51 | 555 | 244 | 714 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777638 | 555 | 1 | 555 | 0 |
HO794536 | 555 | 1 | 555 | 0 |
HO458123 | 386 | 170 | 555 | 0 |
HO458123 | 169 | 1 | 169 | 0 |
HO619167 | 560 | 9 | 555 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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