Basic Information | |
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Species | Populus trichocarpa |
Cazyme ID | Potri.011G103900.1 |
Family | GT20 |
Protein Properties | Length: 972 Molecular Weight: 109529 Isoelectric Point: 6.7732 |
Chromosome | Chromosome/Scaffold: 11 Start: 12654218 End: 12667131 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 86 | 602 | 0 |
RQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVTALLGVKEFEAKWIGWAGVNVPDEVGQKALTEALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNQHYEEGDVVWCHDYHLMYLPKCLKEYNNNMKVGWFLHTPFPSSEIHRTLPSRSDLLRSVLAADLVG FHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAVCLSRIIHLNSPCLFSLSHSILSGVAPQHEQIRFYSCYDNHALSSSCNQFPIGIDSDRFIRA LELPQVQEHIKELKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENSAWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHH LDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQDSKKGVLILSEFAGAAQSLGAGAILVNPWNITEVADSIRQALTMSPEEREKRHRHNFVHV TTHTAQEWAETFVSELD |
Full Sequence |
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Protein Sequence Length: 972 Download |
MPGNQYNGNS TLNSGRVGRL LKIREQRRSI RSAYSNEVTD NHRGTEACEN DLRTREGDSL 60 NNSFIEQYLE GAIAEGYEKL DVRPLRQRLL VVANRLPVSA VRRGEDSWSL EISAGGLVTA 120 LLGVKEFEAK WIGWAGVNVP DEVGQKALTE ALAEKRCIPV FLDEEIVHQY YNGYCNNILW 180 PLFHYLGLPQ EDRLATTRSF QSQFAAYKKA NQMFADVVNQ HYEEGDVVWC HDYHLMYLPK 240 CLKEYNNNMK VGWFLHTPFP SSEIHRTLPS RSDLLRSVLA ADLVGFHTYD YARHFVSACT 300 RILGLEGTPE GVEDQGRLTR VAAVCLSRII HLNSPCLFSL SHSILSGVAP QHEQIRFYSC 360 YDNHALSSSC NQFPIGIDSD RFIRALELPQ VQEHIKELKE RFAGRKVMLG VDRLDMIKGI 420 PQKILAFEKF LEENSAWRDK VVLLQIAVPT RTDVPEYQKL TSQVHEIVGR INGRFGTLTA 480 VPIHHLDRSL DFHALCALYA VTDVALVTSL RDGMNLVSYE FVACQDSKKG VLILSEFAGA 540 AQSLGAGAIL VNPWNITEVA DSIRQALTMS PEEREKRHRH NFVHVTTHTA QEWAETFVSE 600 LDDTVIEAQL RTKQVPPALP EEDAISRYLQ STNRLLILGF NATLTEPADT PGRRADQIKE 660 MELKLHPELK EALTALCSDR KTTIVVLSGS DRKTLDDNFG EYDMWLAAEH GMFLRLTKGE 720 WMTTMPEHLN MEWVDSVKHV FEYFTERTPR SHFERCETSL VWNYKYADVE FGRLQARDML 780 QHLWTGPISN ASVDVVQGNR SVEVRAVGVT KGAAIDRILG EIVHSKSMTT PIDYVLCIGH 840 FLGKDEDVYT FFEPELPSDS LAVARTKQND GLKSPIDRRP SMKLPAIRSV SKSSQGKAQR 900 PLLNPEKRTA NHGCGSGRRP SAEKISWNVL DLKGDNYFSC AVGRTRTNAR YLLGSSDDVV 960 SFLMKLANAS T* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 1 | 971 | 980 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 81 | 971 | 891 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PRK14501 | PRK14501 | 0 | 88 | 864 | 784 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 88 | 603 | 518 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 87 | 601 | 521 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABO61742.1 | 0 | 1 | 969 | 1 | 919 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
RefSeq | NP_177979.1 | 0 | 1 | 971 | 1 | 940 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002285632.1 | 0 | 1 | 971 | 1 | 925 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002331300.1 | 0 | 1 | 971 | 1 | 922 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531237.1 | 0 | 1 | 971 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 88 | 613 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 88 | 613 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 88 | 613 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 88 | 613 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 88 | 613 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
ES813375 | 361 | 498 | 858 | 0 |
CX546763 | 353 | 75 | 426 | 0 |
CV711307 | 342 | 86 | 427 | 0 |
DW069910 | 337 | 174 | 510 | 0 |
CX545499 | 309 | 436 | 744 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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