y
Basic Information | |
---|---|
Species | Physcomitrella patens |
Cazyme ID | Pp1s100_191V6.1 |
Family | GH13 |
Protein Properties | Length: 969 Molecular Weight: 107776 Isoelectric Point: 6.7216 |
Chromosome | Chromosome/Scaffold: 100 Start: 999814 End: 1005839 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
---|
NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH13 | 600 | 884 | 2.2e-40 |
DLSKCGITTIWLPPPTHSVAPQGYMPGDLYNLNSAYGGSEELKQCIDEMHKHNILVLGDVVLNHRCAQKQSPNGVWNRFGGKLNWGPEAIVRDDPNFQGQ GNPKSGDFFHAAPNIDHSQDFVRRDIIEWMKWLRSDFGFDGWRLDFVRGFWGGYVKEYIEATKPAFAIGEYWDSLAYEGGQVSYNQDAHRQRIVNWINAA GGTSSAFDVTTKGILHSALHGEFWRLIDPQGKPPGVMGWWPSRAVTFLENHDTGSTQGHWPFPRDKLMMGYAYILTHPGTPVIFH |
Full Sequence |
---|
Protein Sequence Length: 969 Download |
MFCKYLPLIP ALVQCKRMQD TVSARSLAGM SSNLLSSPAR TWHGEFRLVS QQIPLISSYH 60 VTGSNTNNST SVRTSFFGER SPNTSKVAKG QRRSRPDHAV VSVLKGDRIT GSRDVQAPDD 120 VESLKAALAA AEARTDAAKQ AEKKALDALA AMKNKSRDMA QNDQNSQEVF EGISLAVQVE 180 KISESTIQKA TLRITEDAEL KIAAAETAAA EVILELEDQF RRAAEDAVQA ASVEAQVTID 240 EARAAVSAAR VQAEKSEAIL NKQVKALNEL AEAEAKVLML EEALLDAGRK LQLANGETER 300 IRIELDSAQR FIKTATARAE AAERTAEELQ RAAAKEAEER ADSAQSAINA VKKATQVRLD 360 ADKIAFEAEL DALRSANDTS HKASEARRLV DKSRFELLER SLLAVESATA AWKNRALMAE 420 KLLRLARING AEIDTSSLPV EQAPSVGRLE VLPGSDVRIK DLLENGPRRE TPDWMKRRLQ 480 IGQQVLPPMQ PIAINADVDA LIPLQLPSSE TVWDVSKSKV KENDKYAVRA AEKEALDLQR 540 NAMERALQTK SIKTLVRYPE DAEEKSESGT GSGREIVFQG FNWESWRRQW WLEMSAKASD 600 LSKCGITTIW LPPPTHSVAP QGYMPGDLYN LNSAYGGSEE LKQCIDEMHK HNILVLGDVV 660 LNHRCAQKQS PNGVWNRFGG KLNWGPEAIV RDDPNFQGQG NPKSGDFFHA APNIDHSQDF 720 VRRDIIEWMK WLRSDFGFDG WRLDFVRGFW GGYVKEYIEA TKPAFAIGEY WDSLAYEGGQ 780 VSYNQDAHRQ RIVNWINAAG GTSSAFDVTT KGILHSALHG EFWRLIDPQG KPPGVMGWWP 840 SRAVTFLENH DTGSTQGHWP FPRDKLMMGY AYILTHPGTP VIFHDHFYDF GLHDQIADLI 900 AVRTRTGVHC RSKVKIFQAN FEGYAAQVGD NLVMKIGHLD WNPSKQNNLA GSWNRCTDKG 960 EYQLWERK* 1020 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 2.0e-45 | 575 | 903 | 410 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 4.0e-154 | 575 | 937 | 376 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 1.0e-172 | 576 | 914 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 1.0e-180 | 572 | 967 | 401 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 564 | 968 | 409 | + alpha-amylase |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI21221.1 | 0 | 171 | 968 | 184 | 975 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_001768202.1 | 0 | 578 | 968 | 1 | 391 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001782830.1 | 0 | 577 | 967 | 14 | 404 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_002276872.1 | 0 | 171 | 960 | 184 | 966 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002526120.1 | 0 | 171 | 968 | 185 | 972 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3bsg_A | 0 | 574 | 937 | 1 | 377 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 2qps_A | 0 | 574 | 937 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1rpk_A | 0 | 574 | 937 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1p6w_A | 0 | 574 | 937 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1ht6_A | 0 | 574 | 937 | 1 | 377 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
HO795567 | 626 | 345 | 969 | 0 |
FC372589 | 274 | 423 | 696 | 0 |
FC326597 | 269 | 436 | 704 | 0 |
FC344027 | 258 | 655 | 912 | 0 |
FC342438 | 274 | 385 | 658 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|