PlantCAZyme

Basic Information
Species	Physcomitrella patens
Cazyme ID	Pp1s128_133V6.1
Family	AA7
Protein Properties	Length: 502 Molecular Weight: 54617.5 Isoelectric Point: 9.9712
Chromosome	Chromosome/Scaffold: 128 Start: 969653 End: 972375
Description	FAD-binding Berberine family protein
View CDS

External Links
NCBI Taxonomy
Plaza
CAZyDB

Signature Domain Download full data set without filtering

Family	Start	End	Evalue
AA7	66	498	0
ARLAYNLRERYAPSAFVFPTTVAQVQNAVFCAKQVGVGIVPRGGGHSYEDYSLGGRDGVLVVDMEGFKQFSYNKAAKTAVVGAGFRLGPLYLALWNAGKV TIPAGNCPTVGIAGHALGGGWGFSSRKFGLVTDNILEVQLVAANGTVVTANAQKNKDLYFAIRGAGATSYGIVTQFTFRVHDVSAPVTHFKYRWNDKAVL FKNFKSFQSWGLNVPAEISAAFYMDPSGVSWLEGTYLGKKTSLLPLVKTFLASAAPNPTRVEEELNWIQLILVNWNYPSNTNPNQLNNVPFTTNTFKAKS IYVNGPGLSDAGINAMINAMNTGSNAYFIYDLYGSQSAINKVVPGETAFIHRNSLYSIQMVASWSNDNNAVTQTSYITRYWKVVRTYATGQAYQNYIDRD MPLSAYYGSSLSTLIAGKKKWDPQNVFNFPQSI

Full Sequence
Protein Sequence Length: 502 Download
MGLKFAVLRV TAALACVLMV LLMCFQPVAG NEQRMGRKLA ATTLKQCLAK GGARTAFPGT 60 SEYSTARLAY NLRERYAPSA FVFPTTVAQV QNAVFCAKQV GVGIVPRGGG HSYEDYSLGG 120 RDGVLVVDME GFKQFSYNKA AKTAVVGAGF RLGPLYLALW NAGKVTIPAG NCPTVGIAGH 180 ALGGGWGFSS RKFGLVTDNI LEVQLVAANG TVVTANAQKN KDLYFAIRGA GATSYGIVTQ 240 FTFRVHDVSA PVTHFKYRWN DKAVLFKNFK SFQSWGLNVP AEISAAFYMD PSGVSWLEGT 300 YLGKKTSLLP LVKTFLASAA PNPTRVEEEL NWIQLILVNW NYPSNTNPNQ LNNVPFTTNT 360 FKAKSIYVNG PGLSDAGINA MINAMNTGSN AYFIYDLYGS QSAINKVVPG ETAFIHRNSL 420 YSIQMVASWS NDNNAVTQTS YITRYWKVVR TYATGQAYQN YIDRDMPLSA YYGSSLSTLI 480 AGKKKWDPQN VFNFPQSIPL K* 540

Full Sequence

Protein Sequence Length: 502 Download

MGLKFAVLRV TAALACVLMV LLMCFQPVAG NEQRMGRKLA ATTLKQCLAK GGARTAFPGT    60
SEYSTARLAY NLRERYAPSA FVFPTTVAQV QNAVFCAKQV GVGIVPRGGG HSYEDYSLGG    120
RDGVLVVDME GFKQFSYNKA AKTAVVGAGF RLGPLYLALW NAGKVTIPAG NCPTVGIAGH    180
ALGGGWGFSS RKFGLVTDNI LEVQLVAANG TVVTANAQKN KDLYFAIRGA GATSYGIVTQ    240
FTFRVHDVSA PVTHFKYRWN DKAVLFKNFK SFQSWGLNVP AEISAAFYMD PSGVSWLEGT    300
YLGKKTSLLP LVKTFLASAA PNPTRVEEEL NWIQLILVNW NYPSNTNPNQ LNNVPFTTNT    360
FKAKSIYVNG PGLSDAGINA MINAMNTGSN AYFIYDLYGS QSAINKVVPG ETAFIHRNSL    420
YSIQMVASWS NDNNAVTQTS YITRYWKVVR TYATGQAYQN YIDRDMPLSA YYGSSLSTLI    480
AGKKKWDPQN VFNFPQSIPL K*                                             540

Functional Domains Download unfiltered results here

Cdd ID	Domain	E-Value	Start	End	Length	Domain Description
PLN02805	PLN02805	0.006	78	134	57	+ D-lactate dehydrogenase [cytochrome]
pfam08031	BBE	3.0e-9	457	499	45	+ Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine.
pfam01565	FAD_binding_4	1.0e-18	78	205	130	+ FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan.
COG0277	GlcD	6.0e-20	60	501	475	+ FAD/FMN-containing dehydrogenases [Energy production and conversion]

Gene Ontology
GO Term	Description
GO:0008762	UDP-N-acetylmuramate dehydrogenase activity
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0055114	oxidation-reduction process

Annotations - NR Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
RefSeq	XP_001770677.1	0	1	501	406	906	predicted protein [Physcomitrella patens subsp. patens]
RefSeq	XP_001938664.1	0	44	498	29	490	glucooligosaccharide oxidase [Pyrenophora tritici-repentis Pt-1C-BFP]
RefSeq	XP_002148536.1	0	35	501	1	474	glucooligosaccharide oxidase, putative [Penicillium marneffei ATCC 18224]
RefSeq	YP_001252780.1	0	48	498	1	434	FAD-binding protein [Clostridium botulinum A str. ATCC 3502]
RefSeq	YP_001785568.1	0	48	498	1	434	FAD-binding protein [Clostridium botulinum A3 str. Loch Maree]

Annotations - PDB Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
PDB	3rja_A	0	44	501	3	473	A Chain A, Crystal Structure Of 1,2-A-L-Fucosidase From Bifidobacterium Bifidum (Apo Form)
PDB	3rj8_A	0	44	501	3	473	A Chain A, Crystal Structure Of Carbohydrate Oxidase From Microdochium Nivale
PDB	3tsj_B	8e-35	25	499	2	494	A Chain A, Crystal Structure Of Carbohydrate Oxidase From Microdochium Nivale
PDB	3tsj_A	8e-35	25	499	2	494	A Chain A, Crystal Structure Of Carbohydrate Oxidase From Microdochium Nivale
PDB	3tsh_A	8e-35	25	499	2	494	A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity

Metabolic Pathways
Pathway Name	Reaction	EC	Protein Name
berberine biosynthesis	RETICULINE-OXIDASE-RXN	EC-1.21.3.3	reticuline oxidase
dehydroscoulerine biosynthesis	RETICULINE-OXIDASE-RXN	EC-1.21.3.3	reticuline oxidase
dehydroscoulerine biosynthesis	RXN-10888	EC-1.21.3	reticuline oxidase
sanguinarine and macarpine biosynthesis	RETICULINE-OXIDASE-RXN	EC-1.21.3.3	reticuline oxidase

EST Download unfiltered results here

Hit	Length	Start	End	EValue
FC450930	168	335	502	0
BY953955	125	1	125	0
BY975336	114	1	114	0
GO913670	143	33	175	0
BJ872295	247	236	478	0

Orthologous Group
Species	ID
Physcomitrella patens	Pp1s85_169V6.1

Signal Peptide

Cleavage Site
30

CAZyme Information