Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s238_22V6.1 |
Family | GH32 |
Protein Properties | Length: 422 Molecular Weight: 47713.9 Isoelectric Point: 6.5081 |
Chromosome | Chromosome/Scaffold: 238 Start: 329698 End: 332225 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 37 | 166 | 1.2e-39 |
HFRPEKDWMNGVSSTTCLLSKDPCFYNGFYHFFYQYNPHGAVWGNIVWGHAVSRDMIHWQHLDIALILDKWYDIQGVWSGSVTMREDGVPIILYTGSSYA SEQTQCIANPADPSDPLLRKWVKDHENPIL |
Full Sequence |
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Protein Sequence Length: 422 Download |
MFSREVNYYT DVELRDDYEN NTLRIDAERY LQRPSYHFRP EKDWMNGVSS TTCLLSKDPC 60 FYNGFYHFFY QYNPHGAVWG NIVWGHAVSR DMIHWQHLDI ALILDKWYDI QGVWSGSVTM 120 REDGVPIILY TGSSYASEQT QCIANPADPS DPLLRKWVKD HENPILRFLI QAADVNKGWS 180 SVQAIPRAVW LDRNTLKGLI QDPIEEVKTL RGSKVQQGTV KLAPGEVLGI RGATGRQLDI 240 EVVFEYPDVT HTINSGTFNL DRDLVNCNQG GAAHRGLFGP FGLLVLADEN LREQTAIFFY 300 ISYSRDQGKR ATSFCSDQSR SSLLSDVATT VYGSFVEILP SEDSLSLRVL VDKSIVESFV 360 QGGRMVITSR VYPIIAMNKT SHLYFLNNAT TSINVRSIDV WQMHPAAYGT LQCHRCSAAI 420 R* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
smart00640 | Glyco_32 | 2.0e-32 | 171 | 363 | 197 | + Glycosyl hydrolases family 32. | ||
cd08996 | GH32_B_Fructosidase | 1.0e-41 | 44 | 166 | 129 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
smart00640 | Glyco_32 | 3.0e-49 | 37 | 167 | 136 | + Glycosyl hydrolases family 32. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-52 | 37 | 166 | 133 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_001764172.1 | 0 | 24 | 167 | 26 | 161 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001764172.1 | 0 | 171 | 407 | 325 | 560 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001778419.1 | 0 | 1 | 365 | 1 | 339 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001778420.1 | 0 | 121 | 403 | 32 | 302 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001784437.1 | 0 | 172 | 403 | 279 | 508 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 171 | 418 | 299 | 543 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
PDB | 2aey_A | 4e-32 | 33 | 166 | 8 | 134 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
PDB | 2ade_A | 0 | 171 | 418 | 299 | 543 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
PDB | 2ade_A | 4e-32 | 33 | 166 | 8 | 134 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
PDB | 2add_A | 0 | 171 | 418 | 299 | 543 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DC940328 | 216 | 171 | 386 | 0 |
FC369012 | 234 | 172 | 403 | 0 |
DR094404 | 235 | 171 | 404 | 0 |
CO363936 | 235 | 171 | 404 | 0 |
DR167118 | 229 | 177 | 404 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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