Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s246_61V6.1 |
Family | GT13 |
Protein Properties | Length: 370 Molecular Weight: 42535.1 Isoelectric Point: 5.8514 |
Chromosome | Chromosome/Scaffold: 246 Start: 321695 End: 327790 |
Description | alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, putative |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT13 | 19 | 357 | 0 |
TELEDFQNKEQLAKISGSSKLGPVAAVVIMACNRPDYLERTLKSVTKYHKDVAEKFPLFVSQDGSNQDVMDTARSFSSVNFLQHLDENPPKTRNPGEIIA YYKIASHYKWALTQLFNKRDFRRVVILEDDMEISPDFFSYFEATAPLLDTDKTLLAISSWNDNGQEKFVSDPEQLYRSDFFPGLGWMLTKSTWEELAPKL KENRKGRQVIRPEVCRTYNFGEQGSSLGQFYEQYLKSIKLNNVNVDWRSKDLNYLKKGQFNPIFNSLVSKAAIIPASQALTEANYGEGDIRVQYYSLVEF KYLAHEFGIFEDTKDGIPRTAYNGVIVFRWKGQKRVFLT |
Full Sequence |
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Protein Sequence Length: 370 Download |
MSGVDEKARL EEDNRVLGTE LEDFQNKEQL AKISGSSKLG PVAAVVIMAC NRPDYLERTL 60 KSVTKYHKDV AEKFPLFVSQ DGSNQDVMDT ARSFSSVNFL QHLDENPPKT RNPGEIIAYY 120 KIASHYKWAL TQLFNKRDFR RVVILEDDME ISPDFFSYFE ATAPLLDTDK TLLAISSWND 180 NGQEKFVSDP EQLYRSDFFP GLGWMLTKST WEELAPKLKE NRKGRQVIRP EVCRTYNFGE 240 QGSSLGQFYE QYLKSIKLNN VNVDWRSKDL NYLKKGQFNP IFNSLVSKAA IIPASQALTE 300 ANYGEGDIRV QYYSLVEFKY LAHEFGIFED TKDGIPRTAY NGVIVFRWKG QKRVFLTNFF 360 QQLDTIETL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06532 | Glyco_transf_25 | 0.009 | 112 | 154 | 44 | + Glycosyltransferase family 25 [lipooligosaccharide (LOS) biosynthesis protein] is a family of glycosyltransferases involved in LOS biosynthesis. The members include the beta(1,4) galactosyltransferases: Lgt2 of Moraxella catarrhalis, LgtB and LgtE of Neisseria gonorrhoeae and Lic2A of Haemophilus influenzae. M. catarrhalis Lgt2 catalyzes the addition of galactose (Gal) to the growing chain of LOS on the cell surface. N. gonorrhoeae LgtB and LgtE link Gal-beta(1,4) to GlcNAc (N-acetylglucosamine) and Glc (glucose), respectively. The genes encoding LgtB and LgtE are two genes of a five gene locus involved in the synthesis of gonococcal LOS. LgtE is believed to perform the first step in LOS biosynthesis. | ||
cd00761 | Glyco_tranf_GTA_type | 0.002 | 45 | 161 | 117 | + Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities. | ||
pfam13641 | Glyco_tranf_2_3 | 0.0004 | 44 | 171 | 128 | + Glycosyltransferase like family 2. Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis. | ||
pfam03071 | GNT-I | 1.0e-144 | 7 | 356 | 366 | + GNT-I family. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GNT-I, GLCNAC-T I) EC:2.4.1.101 transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide. This is an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localised to the Golgi apparatus, and is probably distributed in all tissues. The catalytic domain is located at the C-terminus. | ||
cd02514 | GT13_GLCNAC-TI | 6.0e-173 | 42 | 361 | 336 | + GT13_GLCNAC-TI is involved in an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GLCNAC-T I , GNT-I) transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide, an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localized to the Golgi apparatus. The catalytic domain is located at the C-terminus. These proteins are members of the glycosy transferase family 13. |
Gene Ontology | |
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GO Term | Description |
GO:0000139 | Golgi membrane |
GO:0003827 | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity |
GO:0006487 | protein N-linked glycosylation |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAC80697.1 | 0 | 30 | 358 | 96 | 434 | N-acetylglucosaminyltransferase I [Solanum tuberosum] |
EMBL | CAC80699.1 | 0 | 30 | 357 | 23 | 360 | N-acetylglucosaminyltransferase I [Solanum tuberosum] |
EMBL | CAD22107.1 | 0 | 1 | 369 | 92 | 471 | N-acetylglucosaminylaminotransferase I [Physcomitrella patens] |
RefSeq | NP_195537.2 | 0 | 5 | 358 | 70 | 432 | CGL1 (COMPLEX GLYCAN LESS 1); alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase/ protein N-acetylglucosaminyltransferase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_001778887.1 | 0 | 1 | 369 | 54 | 422 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2apc_A | 0 | 42 | 357 | 2 | 329 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
PDB | 2am5_A | 0 | 42 | 357 | 2 | 329 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
PDB | 2am4_A | 0 | 42 | 357 | 2 | 329 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
PDB | 2am3_A | 0 | 42 | 357 | 2 | 329 | A Chain A, Crystal Structure Of N-Acetylglucosaminyltransferase I In Complex With Udp-Glucose |
PDB | 1fo8_A | 0 | 42 | 357 | 3 | 330 | A Chain A, Crystal Structure Of N-Acetylglucosaminyltransferase I |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FC402637 | 252 | 100 | 340 | 0 |
FC402636 | 228 | 154 | 370 | 0 |
BJ594779 | 216 | 166 | 370 | 0 |
BJ170544 | 215 | 167 | 370 | 0 |
GO362875 | 290 | 34 | 312 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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