| Basic Information | |
|---|---|
| Species | Physcomitrella patens |
| Cazyme ID | Pp1s24_272V6.1 |
| Family | GH16 |
| Protein Properties | Length: 582 Molecular Weight: 65273.4 Isoelectric Point: 6.6122 |
| Chromosome | Chromosome/Scaffold: 24 Start: 2592911 End: 2594656 |
| Description | xyloglucan endotransglucosylase/hydrolase 4 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 411 | 553 | 3.1e-22 |
| CGGRLRSLNSYPSGKFSSRIKCAEGDTSGLLTSFYVSSGEGTITQDEIDFEFLGDNKRIVQTNFYVNGTSDNEQWVELDYDCSSGFHTYAIHYDQHQIKW FVDSKLVRTVVKEDQLRERLPYPTKRMFLYSSVWNASFVNDGG | |||
| Full Sequence |
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| Protein Sequence Length: 582 Download |
| MSTPNLASMR IGIAGLQLKE LRRAHIGLEQ HCRLQVEAMQ GALRDSSKKV FDDPNGARRE 60 LVRVQKLVQT CNLNLTAIGQ HLQSHDLLAK VGQSVASAES LIDQSEITGP LPSPQVESVN 120 DEASESVFET EGSAPSTLSS RVSEDRNSRQ TGLTPSINNL GPFPVPPKFE MSMSELVVDF 180 CSNHVIRTLS TAIIRLDRSC GGKFHTTAKY TSGVFTIRMR APANAPGMIN SFYISSNDGA 240 LDMISFDFVG NQPNRVLTAY AVNGNHEQKL KTFHMDFDTT AEFHQYTIKW DNEMIVWMVD 300 DVVLRTLRAS CVKDYPTKPG HIFGYSWDAS SLNDGAMAGR MNWLNAPYFM HFKNLQVTSP 360 LRRGDWVPPQ QKNVQRTSTN SIRPFVIDYC PWSVAADAEH DVDITFDKKG CGGRLRSLNS 420 YPSGKFSSRI KCAEGDTSGL LTSFYVSSGE GTITQDEIDF EFLGDNKRIV QTNFYVNGTS 480 DNEQWVELDY DCSSGFHTYA IHYDQHQIKW FVDSKLVRTV VKEDQLRERL PYPTKRMFLY 540 SSVWNASFVN DGGWTGKWHG MGELPFVARF KDVVAQYFSN E* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02176 | GH16_XET | 2.0e-23 | 196 | 357 | 175 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| cd02175 | GH16_lichenase | 2.0e-30 | 390 | 573 | 196 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-31 | 183 | 343 | 162 | + Glycosyl hydrolases family 16. | ||
| pfam00722 | Glyco_hydro_16 | 8.0e-37 | 404 | 547 | 145 | + Glycosyl hydrolases family 16. | ||
| cd02176 | GH16_XET | 1.0e-38 | 402 | 572 | 182 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_001756795.1 | 5e-16 | 198 | 329 | 7 | 144 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001756795.1 | 0 | 403 | 546 | 1 | 144 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001759442.1 | 0 | 197 | 357 | 1 | 161 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001759442.1 | 8e-17 | 409 | 572 | 2 | 158 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001759443.1 | 0 | 455 | 577 | 2 | 125 | predicted protein [Physcomitrella patens subsp. patens] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2vh9_B | 7e-19 | 412 | 572 | 72 | 231 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2vh9_B | 9e-16 | 194 | 359 | 65 | 236 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2vh9_A | 7e-19 | 412 | 572 | 72 | 231 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2vh9_A | 9e-16 | 194 | 359 | 65 | 236 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2uwb_B | 9e-19 | 412 | 572 | 49 | 208 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BQ040266 | 133 | 422 | 553 | 0 |
| GW598519 | 306 | 276 | 573 | 0 |
| GW598519 | 207 | 157 | 358 | 2e-24 |
| BQ040266 | 132 | 212 | 336 | 0.000000002 |
| GW598519 | 44 | 477 | 518 | 0.7 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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