Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s371_32V6.1 |
Family | GH32 |
Protein Properties | Length: 689 Molecular Weight: 75928.5 Isoelectric Point: 5.4165 |
Chromosome | Chromosome/Scaffold: 371 Start: 171741 End: 175530 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 159 | 480 | 0 |
HFQPDKDWMNDPNGPMYYQGYYHLFYQYNPVGAVWGNIVWGHTVSTDLVHWRHLEPALKGDQWYDIRGIWSGSATTLSDGTPVLLYTGWSEAYDQIQSMA IPVNKSDPLLRQWLKAPQNPMAVVPEGYNSSQFRDPTTAWQGPDGLWRLLVGANTGDGGTIGTALLFKSIDFQAWNFSHSLHSVPGTGMWECPDFYPVAL SGTLLGADTSTHGPTVKHVLKISANDKLHDYYSVGSYITENDTFLPESVNLDAGIGLRYDYGKFYASKSFFDQAKRRRILFGWVNESDSQEANIQKGWAS VMSIPRQVWLDNRTTTDLIQYP |
Full Sequence |
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Protein Sequence Length: 689 Download |
MPQSSDHGPV REGEGEAATL IDTVVPPSDS SSYISVPSAE LDISSERNGN NKPSVAILPH 60 SNKEWQSNAT SYSSIPDHPN TLLATDDGPL ILGAVPFSLD GKHSPTHNGS KITKILLLVA 120 LILLILAVLI AVAVVLQARR AGFKRIMDIR AFKHRTAFHF QPDKDWMNDP NGPMYYQGYY 180 HLFYQYNPVG AVWGNIVWGH TVSTDLVHWR HLEPALKGDQ WYDIRGIWSG SATTLSDGTP 240 VLLYTGWSEA YDQIQSMAIP VNKSDPLLRQ WLKAPQNPMA VVPEGYNSSQ FRDPTTAWQG 300 PDGLWRLLVG ANTGDGGTIG TALLFKSIDF QAWNFSHSLH SVPGTGMWEC PDFYPVALSG 360 TLLGADTSTH GPTVKHVLKI SANDKLHDYY SVGSYITEND TFLPESVNLD AGIGLRYDYG 420 KFYASKSFFD QAKRRRILFG WVNESDSQEA NIQKGWASVM SIPRQVWLDN RTTTDLIQYP 480 VEELNSLRGP EVSSENVTVW AGSFVKLDGI HGNQLDIEIS FAMPNRSVGL QPPELLAESG 540 QLLCNQRGAV GNTKIGPFGV YVLATDDFRE LTAIYFHVLQ SPGEGLKILV CSDQTQSSVA 600 PNLDTASFGS FVRVYESDQY LTLRILVDHS IVETFVQGGR AVVTSRVYPE LALDDAAHVY 660 LFNNGTELVT VMSVRAWNMN SVQIIPFS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG1621 | SacC | 5.0e-63 | 154 | 521 | 382 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-84 | 166 | 483 | 330 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-123 | 159 | 480 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-148 | 159 | 639 | 491 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_001755639.1 | 0 | 154 | 688 | 32 | 571 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001761961.1 | 0 | 154 | 687 | 5 | 536 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001764172.1 | 0 | 149 | 684 | 29 | 561 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001774490.1 | 0 | 154 | 684 | 39 | 582 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001783601.1 | 0 | 147 | 688 | 1 | 542 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 154 | 685 | 7 | 534 | A Chain A, Peanut Peroxidase |
PDB | 2ade_A | 0 | 154 | 685 | 7 | 534 | A Chain A, Peanut Peroxidase |
PDB | 2add_A | 0 | 154 | 685 | 7 | 534 | A Chain A, Peanut Peroxidase |
PDB | 1st8_A | 0 | 154 | 685 | 7 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 154 | 685 | 7 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |