Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s37_120V6.1 |
Family | GH32 |
Protein Properties | Length: 218 Molecular Weight: 24580.1 Isoelectric Point: 9.3829 |
Chromosome | Chromosome/Scaffold: 37 Start: 634075 End: 635153 |
Description | beta-fructofuranosidase 5 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 58 | 187 | 1.7e-25 |
RKWVKAPYNPIAPIPLGYNSSQFRDPTEAWRLFDGVWGLLVGANAGEGGLIGTALLYKSMDFQTWSFSNRLHEDPTTGMWECPDLFPVRIKGRKDLNTSA VGKGVLHVLKVSLELKTKHDYYSVGNYLAE |
Full Sequence |
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Protein Sequence Length: 218 Download |
MLYLLYQHQI GRFTGRQPRL ATIKYREANY NKKDLSSVYP SKVQARGDPE DPSDPLPRKW 60 VKAPYNPIAP IPLGYNSSQF RDPTEAWRLF DGVWGLLVGA NAGEGGLIGT ALLYKSMDFQ 120 TWSFSNRLHE DPTTGMWECP DLFPVRIKGR KDLNTSAVGK GVLHVLKVSL ELKTKHDYYS 180 VGNYLAEANT YKPLIVEINT SIELSRCRSK CESAFMT* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-10 | 61 | 145 | 91 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-15 | 59 | 150 | 94 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-19 | 58 | 184 | 134 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
smart00640 | Glyco_32 | 5.0e-32 | 43 | 193 | 154 | + Glycosyl hydrolases family 32. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-32 | 43 | 193 | 155 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_001755639.1 | 0 | 37 | 204 | 131 | 299 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001761961.1 | 0 | 42 | 204 | 101 | 262 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001774490.1 | 0 | 31 | 204 | 135 | 306 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001783601.1 | 0 | 42 | 204 | 107 | 269 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001784437.1 | 6e-35 | 49 | 194 | 93 | 235 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 3e-28 | 42 | 193 | 107 | 253 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 2ade_A | 3e-28 | 42 | 193 | 107 | 253 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 2add_A | 3e-28 | 42 | 193 | 107 | 253 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 1st8_A | 3e-28 | 42 | 193 | 107 | 253 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2xqr_K | 3e-28 | 42 | 193 | 103 | 256 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FC364548 | 164 | 42 | 204 | 0 |
EX433081 | 145 | 42 | 185 | 9e-36 |
DR479610 | 146 | 49 | 193 | 3e-35 |
CT576449 | 153 | 42 | 193 | 3e-35 |
DR690484 | 153 | 42 | 193 | 7e-35 |
Sequence Alignments (This image is cropped. Click for full image.) |
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