| Basic Information | |
|---|---|
| Species | Physcomitrella patens |
| Cazyme ID | Pp1s410_28V6.1 |
| Family | GH16 |
| Protein Properties | Length: 298 Molecular Weight: 33457.2 Isoelectric Point: 9.2756 |
| Chromosome | Chromosome/Scaffold: 410 Start: 168167 End: 169932 |
| Description | xyloglucan endotransglucosylase/hydrolase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 53 | 212 | 2e-21 |
| LLLNDSRAAYTGMGSKQTWLFGGLGAWIKLPANDSAGTVTTFYMASTGPKHCEFDFEFLGNSSGQPYLLHTNIFVDGIGGREQQIRLWFDPTAAFHYYNF QWNKDLLVLYIDNTAIRMFKNIENIVPGFMYPNKCPMGLYLSIWDGSSWATCGGRVKINW | |||
| Full Sequence |
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| Protein Sequence Length: 298 Download |
| MGSVRVTGCL AIAVLVCLSM VVGGVAQRGI PRDFNTWTPK QTRYSPDGRR VELLLNDSRA 60 AYTGMGSKQT WLFGGLGAWI KLPANDSAGT VTTFYMASTG PKHCEFDFEF LGNSSGQPYL 120 LHTNIFVDGI GGREQQIRLW FDPTAAFHYY NFQWNKDLLV LYIDNTAIRM FKNIENIVPG 180 FMYPNKCPMG LYLSIWDGSS WATCGGRVKI NWNSAPFIAT YQLFRLRGCP ATMGDQASIT 240 KCQNSTYAAP GKSSQTIGTA RTRQLRNIRK NYIHYNYCDD KNRYNETQVP ECKYNVL* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 3.0e-15 | 35 | 202 | 180 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-15 | 36 | 225 | 205 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 1.0e-46 | 33 | 212 | 183 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 9.0e-81 | 13 | 292 | 289 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 3.0e-130 | 34 | 292 | 260 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_001770193.1 | 0 | 62 | 297 | 1 | 234 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001772866.1 | 0 | 32 | 297 | 31 | 295 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001777007.1 | 0 | 9 | 297 | 11 | 300 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001781669.1 | 0 | 63 | 297 | 1 | 234 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001784557.1 | 0 | 1 | 297 | 1 | 297 | predicted protein [Physcomitrella patens subsp. patens] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 36 | 293 | 24 | 273 | A Chain A, 2.35 Angstrom Crystal Structure Of Conserved Hypothetical Protein From Toxoplasma Gondii Me49. |
| PDB | 1un1_A | 0 | 36 | 293 | 24 | 273 | A Chain A, 2.35 Angstrom Crystal Structure Of Conserved Hypothetical Protein From Toxoplasma Gondii Me49. |
| PDB | 1umz_B | 0 | 36 | 293 | 24 | 273 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 36 | 293 | 24 | 273 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 2.00386e-43 | 35 | 292 | 26 | 267 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| Transmembrane Domains | |||||
|---|---|---|---|---|---|
| Start | End | ||||
| 7 | 26 | ||||
| Signal Peptide | |||||
|---|---|---|---|---|---|
| Cleavage Site | |||||
| 26 | |||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FC433223 | 204 | 1 | 202 | 0 |
| BJ166862 | 149 | 150 | 298 | 0 |
| AW476854 | 166 | 80 | 245 | 0 |
| CN202713 | 242 | 10 | 249 | 0 |
| BJ952635 | 192 | 107 | 298 | 0 |
| Orthologous Group | |||||
|---|---|---|---|---|---|
| Species | ID | ||||
| Physcomitrella patens | Pp1s81_246V6.1 | Pp1s122_57V6.1 | Pp1s309_36V6.1 | Pp1s213_129V6.1 | Pp1s486_17V6.1 |
| Pp1s486_14V6.1 | Pp1s141_131V6.1 | ||||
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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