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Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s41_238V6.1 |
Family | GH31 |
Protein Properties | Length: 979 Molecular Weight: 112344 Isoelectric Point: 6.8262 |
Chromosome | Chromosome/Scaffold: 41 Start: 1766549 End: 1770427 |
Description | Glycosyl hydrolases family 31 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 470 | 959 | 0 |
LYGQNPPELIKEYTAAVGRMRELPAWITKGVIVGMQGGHKAVRDVWRKVKEYDVPLSAFWLQDWVGQRKTSVGWQLWWNWEVDRDHYQGWEELVRDFKAC GVKTMTYCNPFIVPTDKKTNRQNDLFTIAQKAGYLVSDTIGATYMIPNTSFEAAMLDITNPETRRWFKHLMYEMVKTGVCGWMADFGESLPFDCCLHSGE DPATAHNKYPEMWAELNREFVEEWEQERLVEKQKRKLQSPVDSSRKAEDFKDSDEDDDDDTLVFFMRAGYRGSPRSATLFWEGDQMVSWGANDGIKSAVT GLLSSGVSGYAFNHSDIGGYCTVDLPFIRYSRTEELLLRWMELNAFSVIFRTHEGNVPKANTQFYSNDTTLRHFTRSAKIYKAWEFYRRQLVKEAAAVGM PVVRHLFLHYPEDKYVQTIVYKQFLVGSEILVVPVLDKGHTQVQAYFPSGDVWEHVWTGHQYRAPATQGLKVWVQAPLGYPAVFVKKDSW |
Full Sequence |
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Protein Sequence Length: 979 Download |
MANGNLPRRI YNPFPGQPRG LHFVRGHLHF NASNLTSRKL FAGKNFLVSW DSEAGCLSVH 60 HRNQQDRTLW ATPRHGGFVY AALGESTTYE SRGSYSLYDN VEMHFTHQTI EDIGTYTEFQ 120 SRNVFEGPNF SRNSKLTKCC NDAAIGIDRC GIDLVNLRSP RAHNDHSEVE VDPDGTTLNR 180 DEGDAIVVLT GCLYTNCKLA KDELRLHDRR RSCEERSQFR ERNNNDEEMD AAWFAVGPDG 240 DDLRAGVRYK LVFSEKRDNQ LGFHVELDSP FRKKAETFFS FKSWRFVESS SELRLCLEKQ 300 SSTFRRLVPE LGCQGRIFSY RLGDVDRCHR GGSFRGLTSE NGSGFHGDDE MQCMPKLNRV 360 LLTYASEAGE KFYGFGEQFS CFDMKGKRVP IMVQEQGIGR GDQPITMAAN LVSYRAGGDW 420 HTTYAPSPHY LTSDMNSLFL EGYEHCVFDL TRPDCVQLQV HAGSMKGRIL YGQNPPELIK 480 EYTAAVGRMR ELPAWITKGV IVGMQGGHKA VRDVWRKVKE YDVPLSAFWL QDWVGQRKTS 540 VGWQLWWNWE VDRDHYQGWE ELVRDFKACG VKTMTYCNPF IVPTDKKTNR QNDLFTIAQK 600 AGYLVSDTIG ATYMIPNTSF EAAMLDITNP ETRRWFKHLM YEMVKTGVCG WMADFGESLP 660 FDCCLHSGED PATAHNKYPE MWAELNREFV EEWEQERLVE KQKRKLQSPV DSSRKAEDFK 720 DSDEDDDDDT LVFFMRAGYR GSPRSATLFW EGDQMVSWGA NDGIKSAVTG LLSSGVSGYA 780 FNHSDIGGYC TVDLPFIRYS RTEELLLRWM ELNAFSVIFR THEGNVPKAN TQFYSNDTTL 840 RHFTRSAKIY KAWEFYRRQL VKEAAAVGMP VVRHLFLHYP EDKYVQTIVY KQFLVGSEIL 900 VVPVLDKGHT QVQAYFPSGD VWEHVWTGHQ YRAPATQGLK VWVQAPLGYP AVFVKKDSWV 960 RQQFIHNLEK ENILEKTW* 1020 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06589 | GH31 | 7.0e-28 | 487 | 843 | 365 | + The enzymes of glycosyl hydrolase family 31 (GH31) occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. In most cases, the pyranose moiety recognized in subsite -1 of the substrate binding site is an alpha-D-glucose, though some GH31 family members show a preference for alpha-D-xylose. Several GH31 enzymes can accommodate both glucose and xylose and different levels of discrimination between the two have been observed. Most characterized GH31 enzymes are alpha-glucosidases. In mammals, GH31 members with alpha-glucosidase activity are implicated in at least three distinct biological processes. The lysosomal acid alpha-glucosidase (GAA) is essential for glycogen degradation and a deficiency or malfunction of this enzyme causes glycogen storage disease II, also known as pompe disease. In the endoplasmic reticulum, alpha-glucosidase II catalyzes the second step in the N-linked oligosaccharide processing pathway that constitutes part of the quality control system for glycoprotein folding and maturation. The intestinal enzymes sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM) play key roles in the final stage of carbohydrate digestion, making alpha-glucosidase inhibitors useful in the treatment of type 2 diabetes. GH31 alpha-glycosidases are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively. | ||
pfam01055 | Glyco_hydro_31 | 4.0e-69 | 469 | 958 | 502 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
COG1501 | COG1501 | 1.0e-75 | 372 | 958 | 601 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
cd06594 | GH31_glucosidase_YihQ | 4.0e-168 | 487 | 843 | 358 | + YihQ is a bacterial alpha-glucosidase with a conserved glycosyl hydrolase family 31 (GH31) domain that catalyzes the release of an alpha-glucosyl residue from the non-reducing end of alpha-glucoside substrates such as alpha-glucosyl fluoride. Orthologs of YihQ that have not yet been functionally characterized are present in plants and fungi. YihQ has sequence similarity to other GH31 enzymes such as CtsZ, a 6-alpha-glucosyltransferase from Bacillus globisporus, and YicI, an alpha-xylosidase from Echerichia coli. In bacteria, YihQ (along with YihO) is important for bacterial O-antigen capsule assembly and translocation. | ||
PRK10426 | PRK10426 | 0 | 356 | 958 | 604 | + alpha-glucosidase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI30134.1 | 0 | 8 | 974 | 46 | 905 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_001760287.1 | 0 | 354 | 978 | 1 | 608 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_002266626.1 | 0 | 8 | 974 | 12 | 871 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002308887.1 | 0 | 8 | 974 | 12 | 875 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002522166.1 | 0 | 8 | 974 | 12 | 874 | alpha-xylosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 4ba0_A | 4e-32 | 358 | 960 | 140 | 672 | A Chain A, Barley 1,3-1,4-Beta-Glucanase In Monoclinic Space Group |
PDB | 4b9z_A | 4e-32 | 358 | 960 | 140 | 672 | A Chain A, Barley 1,3-1,4-Beta-Glucanase In Monoclinic Space Group |
PDB | 4b9y_A | 4e-32 | 358 | 960 | 140 | 672 | A Chain A, Crystal Structure Of Apo Agd31b, Alpha-transglucosylase In Glycoside Hydrolase Family 31 |
PDB | 2f2h_F | 2e-25 | 368 | 926 | 158 | 639 | A Chain A, Structure Of The Yici Thiosugar Michaelis Complex |
PDB | 2f2h_E | 2e-25 | 368 | 926 | 158 | 639 | A Chain A, Structure Of The Yici Thiosugar Michaelis Complex |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DY975686 | 302 | 556 | 857 | 0 |
CO467346 | 272 | 413 | 683 | 0 |
FR600285 | 243 | 734 | 974 | 0 |
DT522737 | 247 | 730 | 974 | 0 |
CF211370 | 231 | 730 | 958 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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