Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s49_209V6.1 |
Family | CBM20 |
Protein Properties | Length: 583 Molecular Weight: 63164.1 Isoelectric Point: 9.102 |
Chromosome | Chromosome/Scaffold: 49 Start: 1649481 End: 1652041 |
Description | Carbohydrate-binding-like fold |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM20 | 152 | 235 | 2.1e-27 |
KVKFQLQRECQFGQQFKVVGSGSHFGDWDPSAALPLNWSEGHLWTTEVDIPKDRKFEWKYILVSTEGEETEWQSGPNHVLETVP |
Full Sequence |
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Protein Sequence Length: 583 Download |
MMIMAAIAGP HRAGLLAYTG NGVEAENYFN GIRLVQTVKV GSSVQDRVLR QRCNLIRLAG 60 LSGSCVQSAR QWFRVTAFSE VASSCLYPSF SLLRKITNSF RRLCFIGFRN PVLKILNHEV 120 YRLYHRRRRI IPLLAIDRAL EAVDESSGVT MKVKFQLQRE CQFGQQFKVV GSGSHFGDWD 180 PSAALPLNWS EGHLWTTEVD IPKDRKFEWK YILVSTEGEE TEWQSGPNHV LETVPGASSL 240 LVSIPWERIP HSADSTSDQF QEVQRCEHLE GQDTASDTED LDAIGKDSKV NTSVEKLTEA 300 AASAAGDVLK AGIKAATIVA ASLDATPVEE LLGLAEHADG SLLFSFSGST TTADAAAQPT 360 IQQIESANVN VPTQTLTPAA LPQRHPSSFQ KKSPIPAARS RRTASKAEAD TAELVAAEDK 420 ETPVDSTVSS ISARPERHAS SFSRKSPIPK NRERSSVSSS KRQSSAEATP VAKAPSKSKF 480 TTRSPISRNQ NSSRATRSEK KKSHSSEVAP AELVSPSKTA SADTEVAESE ALEHGAVSKD 540 LSSYKLSELR SMAKAKGLKG YSKLKKGELV KLIAASDVSS EI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd02853 | E_set_MTHase_like_N | 1.0e-13 | 153 | 246 | 94 | + N-terminal Early set domain associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (also called Glycosyltrehalose trehalohydrolase) and similar proteins. E or "early" set domains are associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (MTHase) and similar proteins at the N-terminal end. This subfamily also includes bacterial alpha amylases and 1,4-alpha-glucan branching enzymes which are highly similar to MTHase. Maltooligosyl trehalose synthase (MTSase) and MTHase work together to produce trehalose. MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. The N-terminal domain of MTHase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
pfam00686 | CBM_20 | 5.0e-18 | 153 | 244 | 95 | + Starch binding domain. | ||
smart01065 | CBM_2 | 2.0e-18 | 152 | 233 | 86 | + Starch binding domain. | ||
cd02861 | E_set_pullulanase_like | 2.0e-21 | 153 | 233 | 82 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
cd05467 | CBM20 | 2.0e-30 | 152 | 246 | 98 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. |
Gene Ontology | |
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GO Term | Description |
GO:0003715 | transcription termination factor activity |
GO:0003824 | catalytic activity |
GO:0005975 | carbohydrate metabolic process |
GO:0006353 | DNA-dependent transcription, termination |
GO:2001070 | starch binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK25218.1 | 9e-26 | 148 | 297 | 107 | 246 | unknown [Picea sitchensis] |
RefSeq | XP_001761619.1 | 0 | 1 | 582 | 1 | 531 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001761620.1 | 0 | 326 | 582 | 220 | 525 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001768545.1 | 3.9937e-43 | 1 | 259 | 1 | 212 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001770618.1 | 0 | 4 | 296 | 1 | 257 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2vn7_A | 0.00000005 | 150 | 240 | 496 | 588 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |
PDB | 2vn4_A | 0.00000005 | 150 | 240 | 496 | 588 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
PDB | 1kum_A | 0.0000003 | 151 | 246 | 7 | 107 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
PDB | 1kul_A | 0.0000003 | 151 | 246 | 7 | 107 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
PDB | 1acz_A | 0.0000003 | 151 | 246 | 7 | 107 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FC430875 | 187 | 1 | 187 | 0 |
FC430874 | 231 | 353 | 583 | 0 |
DC911534 | 260 | 7 | 260 | 0 |
FC406088 | 245 | 392 | 583 | 0 |
FC430875 | 19 | 186 | 204 | 0.043 |
Sequence Alignments (This image is cropped. Click for full image.) |
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