| Basic Information | |
|---|---|
| Species | Physcomitrella patens |
| Cazyme ID | Pp1s74_19V6.1 |
| Family | GH16 |
| Protein Properties | Length: 437 Molecular Weight: 48877.6 Isoelectric Point: 8.8653 |
| Chromosome | Chromosome/Scaffold: 74 Start: 101708 End: 104084 |
| Description | xyloglucan endotransglucosylase/hydrolase 30 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 89 | 262 | 1.2e-23 |
| DSSVNDGSEIRLRLEGHSGSGIKSQNSVFRGFFSAGIKLPCGFGGNSAGIVSAFYASNGGSYPSNHDEIDLEFLGVRPGHPYVIQTNIYADGSTGTGREE RFNLWFDPTVGFHNYSILWTYHHIVFFVDDIPIRRYVSRPELGVPYPTKPMNAFATIWDGSTWATEGGQYHVNY | |||
| Full Sequence |
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| Protein Sequence Length: 437 Download |
| MRRFCRIKNG VNGMGITIEF SRLIYSPAYR SCNVVCHVLV LQMAISATAA LQRAHPPVVD 60 VSVFSHVLEV NSSKKAQFVP MFGEPGDIDS SVNDGSEIRL RLEGHSGSGI KSQNSVFRGF 120 FSAGIKLPCG FGGNSAGIVS AFYASNGGSY PSNHDEIDLE FLGVRPGHPY VIQTNIYADG 180 STGTGREERF NLWFDPTVGF HNYSILWTYH HIVFFVDDIP IRRYVSRPEL GVPYPTKPMN 240 AFATIWDGST WATEGGQYHV NYTQGPFDAT FTNLKLEGCV WDPRLQIAAP ECALHTYKAW 300 FNDNAMQEMS AKQMIALEWA RSNFMWYSYC DDLERWPVQP PPECPKKLVI ELHNYICDDS 360 VSLAPATGNL NNLVTSGTPP SPRKGPWRKT PKSKPFARHQ PPTLPRVPGQ KHKRKIGSRT 420 KLDAFHQLPF SLWKTK* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00413 | Glyco_hydrolase_16 | 1.0e-19 | 91 | 256 | 176 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 8.0e-22 | 107 | 277 | 184 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 2.0e-58 | 94 | 263 | 170 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 6.0e-65 | 94 | 347 | 255 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 7.0e-131 | 77 | 344 | 269 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABK24235.1 | 0 | 78 | 353 | 50 | 322 | unknown [Picea sitchensis] |
| RefSeq | XP_001752695.1 | 0 | 101 | 348 | 46 | 293 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_001765152.1 | 0 | 107 | 344 | 1 | 238 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_002263411.1 | 0 | 39 | 347 | 12 | 313 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002320268.1 | 0 | 34 | 344 | 8 | 311 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2uwc_B | 0 | 94 | 335 | 35 | 263 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwc_A | 0 | 94 | 335 | 35 | 263 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwa_C | 0 | 94 | 335 | 38 | 266 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_B | 0 | 94 | 335 | 38 | 266 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_A | 0 | 94 | 335 | 38 | 266 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DC950928 | 276 | 163 | 436 | 0 |
| DC954100 | 201 | 238 | 436 | 0 |
| DC955808 | 201 | 238 | 436 | 0 |
| CO362798 | 256 | 92 | 347 | 0 |
| CO159857 | 246 | 95 | 340 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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