| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb0067s002110.1 |
| Family | GH32 |
| Protein Properties | Length: 543 Molecular Weight: 60367.3 Isoelectric Point: 9.7728 |
| Chromosome | Chromosome/Scaffold: 67 Start: 28883 End: 31305 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 4 | 329 | 0 |
| APFYYKGWYHFFYQYNPKGAVWGNIVWAHSVSRDLINWMALEPAIKPSIPSDKYGCWSGSATTMPDGTPVIMYTGIDRPNTNYQVQNVAYPRNKSDPLLR EWVKPSYNPVIVPEGGINATQFRDPTTAWRATAGGDGHWRLLIGSVRTTTTTGATAPPRGVAYVYRSRDFRRWTRVRRPLHSAATGMWECPDFYPVSSDE DGRRRRVGLETSVPSGARVKHVLKNSLDLRRYDYYTVGTYDRDAERYVPDDPAGDDDGERRLRYDYGNFYASKTFYDPAKRRRILWGWANESDTAADDVA KGWAGIQAIPRTVWLDPSGKQLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 543 Download |
| MHAAPFYYKG WYHFFYQYNP KGAVWGNIVW AHSVSRDLIN WMALEPAIKP SIPSDKYGCW 60 SGSATTMPDG TPVIMYTGID RPNTNYQVQN VAYPRNKSDP LLREWVKPSY NPVIVPEGGI 120 NATQFRDPTT AWRATAGGDG HWRLLIGSVR TTTTTGATAP PRGVAYVYRS RDFRRWTRVR 180 RPLHSAATGM WECPDFYPVS SDEDGRRRRV GLETSVPSGA RVKHVLKNSL DLRRYDYYTV 240 GTYDRDAERY VPDDPAGDDD GERRLRYDYG NFYASKTFYD PAKRRRILWG WANESDTAAD 300 DVAKGWAGIQ AIPRTVWLDP SGKQLLQWPV EEVEALRGKA VTLKNRVIKP GQHVEVTGIQ 360 TAQADVEVSF EVSPSALAGA ERLDPALADD AERLCGVKRA DVKGGVGPFG LWVLASANLK 420 ERTAVFFRVF KAAAGSSNNK PVVLMCTDPT KSSLNPNLYR PTFAGFVDTD ISNGKISLRS 480 LIDRSVVESF GAGGKTCILS RVYPSLAIGN NARLYVFNNG KADVRVSRLT AWEMKKPLMN 540 GA* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-38 | 7 | 345 | 360 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-48 | 7 | 505 | 508 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-83 | 5 | 332 | 338 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-133 | 5 | 329 | 333 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-172 | 5 | 494 | 498 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABM65156.1 | 0 | 7 | 542 | 1 | 536 | cell wall invertase [Sorghum bicolor] |
| GenBank | ABM65159.1 | 0 | 7 | 542 | 1 | 529 | cell wall invertase [Sorghum bicolor] |
| GenBank | ACG47298.1 | 0 | 4 | 542 | 71 | 594 | beta-fructofuranosidase, insoluble isoenzyme 2 precursor [Zea mays] |
| RefSeq | XP_002489106.1 | 0 | 6 | 542 | 1 | 529 | hypothetical protein SORBIDRAFT_0067s002240 [Sorghum bicolor] |
| RefSeq | XP_002489110.1 | 0 | 1 | 542 | 1 | 542 | hypothetical protein SORBIDRAFT_0067s002110 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qqw_A | 0 | 4 | 534 | 22 | 531 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 4 | 534 | 22 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 4 | 534 | 22 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 4 | 534 | 22 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 4 | 534 | 22 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 540 | 4 | 543 | 0 |
| DV857772 | 313 | 221 | 532 | 0 |
| CX611907 | 241 | 303 | 543 | 0 |
| EG399507 | 275 | 204 | 477 | 0 |
| EG398158 | 261 | 223 | 482 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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