| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb01g019000.1 |
| Family | AA7 |
| Protein Properties | Length: 529 Molecular Weight: 58479.7 Isoelectric Point: 6.5927 |
| Chromosome | Chromosome/Scaffold: 1 Start: 19986326 End: 19990787 |
| Description | cytokinin oxidase/dehydrogenase 6 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 52 | 244 | 1.6e-25 |
| VFHSPAAVLRPQSSGDISMLLSFLSGSSLSKVTVAARGAGHSIHGQAQALDGIVVEMRALPAEMEFHRGGEEGQVSYADVSGGVMWIELLEQSLKLGLAP RSWTDYLYLTVGGTLSNAGISGQTFKHGPQISNVLQLEVVTGRGEIVKCSPSKDADLFNAVLGGLGQFGIITRARILLQEAPQKVTWVRAFYD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 529 Download |
| MEVAMVSTRA SLLILILSLC SPYKFIQSPM DFGPLNLLPT TTTASSDFGR IVFHSPAAVL 60 RPQSSGDISM LLSFLSGSSL SKVTVAARGA GHSIHGQAQA LDGIVVEMRA LPAEMEFHRG 120 GEEGQVSYAD VSGGVMWIEL LEQSLKLGLA PRSWTDYLYL TVGGTLSNAG ISGQTFKHGP 180 QISNVLQLEV VTGRGEIVKC SPSKDADLFN AVLGGLGQFG IITRARILLQ EAPQKVTWVR 240 AFYDDFSIFT RDQELLVSIP DLDYVEGFIV LNEQSLHSSS IAFPASVDFN PDFGTKNSPK 300 IYYCIEFAVH DYQHKNTNVH HVVEVISRQM SHMVSQLYSV EVSYFDFLNR VRMEEMSLRS 360 VGLWEVHHPW LNMFVPKAGV ADFRDLLMDN ISPDSFEGLI LIYPLLRDKW DTNTSVVIPD 420 SGSTDRVMYV VGILRSANPE DGCSHHCLQE LLRRHRRIAD AAGARLGAKQ YLGHHPTPAG 480 WHQHFGRRWE QFAERKARFD PLRILGPGQG IFPRTPDAAA YAGISSAF* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 7.0e-8 | 89 | 227 | 139 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 1.0e-21 | 56 | 201 | 147 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 5.0e-26 | 56 | 511 | 466 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 2.0e-128 | 233 | 512 | 288 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 519 | 538 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE05712.2 | 0 | 1 | 520 | 1 | 531 | OSJNBb0065J09.8 [Oryza sativa (japonica cultivar-group)] |
| GenBank | EAY82296.1 | 0 | 1 | 528 | 1 | 526 | hypothetical protein OsI_37507 [Oryza sativa Indica Group] |
| GenBank | EAZ19670.1 | 0 | 1 | 409 | 1 | 409 | hypothetical protein OsJ_35246 [Oryza sativa Japonica Group] |
| RefSeq | NP_001064886.1 | 0 | 1 | 528 | 1 | 526 | Os10g0483500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002467062.1 | 0 | 1 | 528 | 1 | 528 | hypothetical protein SORBIDRAFT_01g019000 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 44 | 515 | 50 | 523 | A Chain A, The Structure Of Alpha-N-Acetylgalactosaminidase |
| PDB | 2exr_A | 0 | 44 | 515 | 50 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 1w1s_A | 0 | 41 | 512 | 54 | 533 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 1w1r_A | 0 | 41 | 512 | 54 | 533 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 1w1q_A | 0 | 41 | 512 | 54 | 533 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
