| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb01g050070.1 |
| Family | GH16 |
| Protein Properties | Length: 341 Molecular Weight: 37890 Isoelectric Point: 7.5214 |
| Chromosome | Chromosome/Scaffold: 1 Start: 73011904 End: 73013544 |
| Description | xyloglucan endotransglucosylase/hydrolase 32 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 98 | 251 | 1.5e-23 |
| KMALTLLMDRTSGSGFKSKRSYRNGYFGVSIKVQPGYTAGVNTAFYLSNNELYPGKHDEIDIELLGTVPGEPYTLQTNVYVRGTGDGAHLVGREMRFHLW FDPTADFHHYAILWNPDEIVFLVDDVPIRRYAAASTTLFPDREMWAYGSIWDAS | |||
| Full Sequence |
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| Protein Sequence Length: 341 Download |
| MHIALSVCVT ACKLKQQLLL DRGMMSERNL LARRQPLPPA IAVAVVVVAV LVHPRAVAVA 60 QAQPSPGYFP SATVRSMAFS EDYDNLWGPQ HQTLSQDKMA LTLLMDRTSG SGFKSKRSYR 120 NGYFGVSIKV QPGYTAGVNT AFYLSNNELY PGKHDEIDIE LLGTVPGEPY TLQTNVYVRG 180 TGDGAHLVGR EMRFHLWFDP TADFHHYAIL WNPDEIVFLV DDVPIRRYAA ASTTLFPDRE 240 MWAYGSIWDA SDWATDGGRY RADYRYQPFL AGFRTFRTAG CEAAAPAAGC PPVPASPAGA 300 GLSVQQRDAM RWAQQRSMVY YYCQDYTKDR SLYPECSLAT * 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 3.0e-22 | 81 | 268 | 202 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd00413 | Glyco_hydrolase_16 | 7.0e-26 | 87 | 259 | 180 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| PLN03161 | PLN03161 | 2.0e-59 | 79 | 337 | 270 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-75 | 79 | 266 | 188 | + Glycosyl hydrolases family 16. | ||
| cd02176 | GH16_XET | 3.0e-107 | 78 | 336 | 267 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACS13755.1 | 0 | 30 | 336 | 3 | 303 | xyloglucan xyloglucosyl transferase [Hordeum vulgare] |
| RefSeq | NP_001048699.1 | 0 | 38 | 336 | 5 | 310 | Os03g0108300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001132709.1 | 0 | 54 | 340 | 19 | 301 | hypothetical protein LOC100194192 [Zea mays] |
| RefSeq | NP_001148998.1 | 0 | 53 | 340 | 17 | 297 | xyloglucan endotransglucosylase/hydrolase protein 32 [Zea mays] |
| RefSeq | XP_002466013.1 | 0 | 1 | 340 | 1 | 340 | hypothetical protein SORBIDRAFT_01g050070 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2uwa_C | 0 | 58 | 336 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_B | 0 | 58 | 336 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_A | 0 | 58 | 336 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwc_B | 0 | 61 | 336 | 1 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwc_A | 0 | 61 | 336 | 1 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EC873456 | 217 | 62 | 278 | 0 |
| DT942787 | 213 | 62 | 274 | 0 |
| ES296343 | 202 | 63 | 264 | 0 |
| CK209892 | 218 | 61 | 278 | 0 |
| HX164462 | 212 | 61 | 272 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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