| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb02g010060.1 |
| Family | GH31 |
| Protein Properties | Length: 693 Molecular Weight: 76164.1 Isoelectric Point: 5.1407 |
| Chromosome | Chromosome/Scaffold: 2 Start: 14736385 End: 14755318 |
| Description | heteroglycan glucosidase 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 393 | 531 | 1.19993e-41 |
| GQPFSGPDIGGFAGNATPKLFGRWMGMGALFPFSRGHTITGSNDHEPWSFGEECEEVCRLALLRRYRLLPHIYTLFYHSHTKGIPVATPVFFADPQDIDL RKVETSFLLGPLLVCASTVPNKGAHECAHILPKGIWLPF | |||
| GH31 | 193 | 392 | 0 |
| VGTVSMPPKWSLGYHQCRSYMSSEKVLKVVRTFREKGIPCDVIWMDIDYMDGFRCFTFDSNRFYNPKSMVDDLHSIGCKAIWMLDPGIKNEEGYFVCESG SEMDVWVQKADGSPFIGKVWPGDCVFPDFTSKRARAWWASLVRDFITNGVDGIWNDMNEPAVADVYGMLMARSTYEGMVLADASKRPFVLTRAGFIGSQR | |||
| Full Sequence |
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| Protein Sequence Length: 693 Download |
| MAAAAGDMVW VRVLEEGVFR FDASEAARAA AGPSLSFADP RRREVPREGS DAPAVLPTCE 60 VVGGVQKVVV KLPSGTSFYG TGEASGPLER TGKQVFTWNT DAWAYGPGTT SLYQSHPWVL 120 AVLPDGKALG VLADTTMPCE FTMIQIDLRQ ESTIKFSVSS AYPVITFGPL NTPAEVITSL 180 SHAIVKMAEF SNVGTVSMPP KWSLGYHQCR SYMSSEKVLK VVRTFREKGI PCDVIWMDID 240 YMDGFRCFTF DSNRFYNPKS MVDDLHSIGC KAIWMLDPGI KNEEGYFVCE SGSEMDVWVQ 300 KADGSPFIGK VWPGDCVFPD FTSKRARAWW ASLVRDFITN GVDGIWNDMN EPAVADVYGM 360 LMARSTYEGM VLADASKRPF VLTRAGFIGS QRGQPFSGPD IGGFAGNATP KLFGRWMGMG 420 ALFPFSRGHT ITGSNDHEPW SFGEECEEVC RLALLRRYRL LPHIYTLFYH SHTKGIPVAT 480 PVFFADPQDI DLRKVETSFL LGPLLVCAST VPNKGAHECA HILPKGIWLP FDFLDSHPDL 540 PVLYLQGGAI LPIGLPIQHV GEASLEDDMS LIIALDENGK AEGVLFEDAG DGYAFTEGDY 600 LLTYYIAELH SSVVTVKVLK SEGSWKRPKR NLKINILVGG SAMISTDGVD GEEIHLTMPS 660 ESEVSSLIAT SELEHKKRFG ISPVTYLWLY CF* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06602 | GH31_MGAM_SI_GAA | 1.0e-97 | 198 | 489 | 332 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
| COG1501 | COG1501 | 5.0e-114 | 72 | 654 | 651 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| pfam01055 | Glyco_hydro_31 | 2.0e-129 | 171 | 551 | 445 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| cd06604 | GH31_glucosidase_II_MalA | 4.0e-151 | 194 | 475 | 339 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| PLN02763 | PLN02763 | 0 | 5 | 680 | 735 | + hydrolase, hydrolyzing O-glycosyl compounds | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAG72144.1 | 0 | 1 | 684 | 1 | 730 | alpha-glucosidase like protein [Hordeum vulgare subsp. vulgare] |
| GenBank | EEC81922.1 | 0 | 8 | 679 | 1 | 717 | hypothetical protein OsI_25772 [Oryza sativa Indica Group] |
| GenBank | EEE67050.1 | 0 | 8 | 679 | 1 | 717 | hypothetical protein OsJ_23995 [Oryza sativa Japonica Group] |
| RefSeq | NP_001059483.1 | 0 | 1 | 679 | 1 | 724 | Os07g0421300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002459763.1 | 0 | 1 | 692 | 1 | 692 | hypothetical protein SORBIDRAFT_02g010060 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ctt_A | 0 | 67 | 635 | 167 | 814 | A Chain A, Crystal Structure Of Udp-Glucosyltransferase Gtfb |
| PDB | 2qmj_A | 0 | 67 | 635 | 167 | 814 | A Chain A, Crystal Structure Of Udp-Glucosyltransferase Gtfb |
| PDB | 2qly_A | 0 | 67 | 635 | 167 | 814 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
| PDB | 3l4z_A | 0 | 67 | 635 | 167 | 814 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
| PDB | 3l4y_A | 0 | 67 | 635 | 167 | 814 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| JG948140 | 299 | 359 | 628 | 0 |
| JG929829 | 291 | 359 | 620 | 0 |
| CO447522 | 250 | 436 | 684 | 0 |
| FL800714 | 241 | 393 | 633 | 0 |
| GO882018 | 305 | 338 | 613 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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