| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb03g027310.1 |
| Family | GH13 |
| Protein Properties | Length: 907 Molecular Weight: 104127 Isoelectric Point: 6.2549 |
| Chromosome | Chromosome/Scaffold: 3 Start: 54797275 End: 54800709 |
| Description | Alpha amylase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 427 | 691 | 3e-24 |
| FQEFTSKVLPHIKKAGYNAVQLIGVVEHKDYSSIGYKVTNYFAVSSRFGTPDDFKKLVDEAHGLGLVVLLDIVHSYASSDELVGLSLYDGSNDCYFHSGK RGHHKYWGTRMFKYDDVDVLHFLLSNLNWWVTEYRIDGFQFHSLSSMLYTHNGFSTFTGAMEEYCNQYVDKDALIYLILANEMLHQLHPDIITIAEDATF YPGLCEPTTQGGLGFDYWVNLSVPEMWLWHLENVPEREWSMNKIIKVLVSSNQNMLSYAENHNQS | |||
| Full Sequence |
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| Protein Sequence Length: 907 Download |
| MTSCPLFLLV PRPPPPLVGA DGCPTSAGRA HLPPSCRPHP GRRHGYPFRC DSSSSSPAPR 60 ERPPRSRQQR QQRPGGRGDA VDPVGFLAKL GVSDRAFAQF LRDRHKALKD RRWEVCSRFI 120 DLKEASSGFE LLGMHRHRQH RIDFMEWAPG ARYCSLVGDF NQWSPTENCA REGHLGHDDF 180 GYWFIILEDK LREGQEQDEY FFQEYNYVDD YDKGDNGVDA EEIMSRMKEE YWEPGEIRSR 240 QSQLEMVVKL YEQMFGPNGP QTEEELGEIP DAQTRYNEFK ASEKADSLSM WPSYDIIDNG 300 QPFDIFNVVT DRASFEKFQA KKPPLAYWVE MRKGRIAWLE KYVPTISHKD KYRVYFNTPD 360 GALERVPAWA TYVLPDAEGK QSYAVHWEPP PEEIYKWRFG RPKVKGSLRI YECHVGISGS 420 EQKVSSFQEF TSKVLPHIKK AGYNAVQLIG VVEHKDYSSI GYKVTNYFAV SSRFGTPDDF 480 KKLVDEAHGL GLVVLLDIVH SYASSDELVG LSLYDGSNDC YFHSGKRGHH KYWGTRMFKY 540 DDVDVLHFLL SNLNWWVTEY RIDGFQFHSL SSMLYTHNGF STFTGAMEEY CNQYVDKDAL 600 IYLILANEML HQLHPDIITI AEDATFYPGL CEPTTQGGLG FDYWVNLSVP EMWLWHLENV 660 PEREWSMNKI IKVLVSSNQN MLSYAENHNQ SISGRKSFAE IILNSGECSV GSVDDNLIRA 720 SSLLKIIKLI TFTTSGGAYL NFMGNEFAHP ERVEFPMPSN DYSFQFANRQ WELLDKGFHK 780 HVFNFDKDVM SLDENERIIS RGSPNILHCD DTSMVISFTR GPFLFVFNFN PEVSHQSYHV 840 GVEEAGEYQL IFNTDETKYG GCGTLKSSQY MRRTCDKRAD GCRNSLELAL PSRSAQVYKL 900 VRILRI* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02447 | PLN02447 | 3.0e-10 | 95 | 189 | 95 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 2.0e-168 | 391 | 787 | 409 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 1 | 906 | 910 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 0 | 80 | 906 | 832 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 343 | 901 | 574 | + 1,4-alpha-glucan-branching enzyme | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI26672.1 | 0 | 38 | 906 | 32 | 896 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_001057611.1 | 0 | 1 | 906 | 1 | 903 | Os06g0367100 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001108121.1 | 0 | 1 | 906 | 1 | 899 | starch branching enzyme III [Zea mays] |
| RefSeq | NP_001154629.1 | 0 | 80 | 906 | 68 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
| RefSeq | XP_002455921.1 | 0 | 1 | 906 | 1 | 906 | hypothetical protein SORBIDRAFT_03g027310 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 344 | 901 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 344 | 901 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 344 | 901 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| JG953387 | 280 | 515 | 794 | 0 |
| EE022432 | 241 | 493 | 733 | 0 |
| FE606762 | 240 | 135 | 374 | 0 |
| JG636329 | 270 | 352 | 621 | 0 |
| BF272517 | 278 | 365 | 642 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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