| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb04g000620.1 |
| Family | GH32 |
| Protein Properties | Length: 488 Molecular Weight: 53384.6 Isoelectric Point: 4.6567 |
| Chromosome | Chromosome/Scaffold: 4 Start: 440298 End: 443139 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 2 | 277 | 0 |
| VPDQWYDTNGVWTGSATTLPDGRLAMLYTGSTNASVQVQCLAVPADDADPLLTNWTKYEGNPVLYPPPGIGPKDFRDPTTAWFDPSDNTWRIVIGSKDDA EGDHAGIAVVYRTKDFVSFELLPGLLHRVARTGMWECIDFYPVATRGKASGNGVDMSDAFGKNGAIVGDVVHVMKASMDDDRHDYYALGRYDAATNEWTP LDAEKDVGIGLRYDWGKFYASKTFYDPAKRRRVLWGWVGETDSERADVSKGWASLQGIPRTVLLDTKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 488 Download |
| MVPDQWYDTN GVWTGSATTL PDGRLAMLYT GSTNASVQVQ CLAVPADDAD PLLTNWTKYE 60 GNPVLYPPPG IGPKDFRDPT TAWFDPSDNT WRIVIGSKDD AEGDHAGIAV VYRTKDFVSF 120 ELLPGLLHRV ARTGMWECID FYPVATRGKA SGNGVDMSDA FGKNGAIVGD VVHVMKASMD 180 DDRHDYYALG RYDAATNEWT PLDAEKDVGI GLRYDWGKFY ASKTFYDPAK RRRVLWGWVG 240 ETDSERADVS KGWASLQGIP RTVLLDTKTG SNLLQWPVEE AETLRTNSTD LSGITIDYGS 300 AFPLNLRRAT QLDIEAEFQL DRRAVMSLNE ADVGYNCSTS GGAAARGALG PFGLLVLADQ 360 HLREQTAVYF YVAKGLDGSL TTHFCQDESR SSSANDIVKR VVGSSVPVLD DETTLSLRVL 420 VDHSIVESFA QGGRSTATSR VYPTEAIYAN AGVFLFNNAT AARVTAKKLV VHEMDSSYNH 480 DYMVTDI* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-31 | 3 | 443 | 457 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-39 | 3 | 459 | 482 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-57 | 1 | 280 | 285 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-106 | 1 | 277 | 284 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-141 | 1 | 433 | 442 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAP59436.1 | 0 | 1 | 487 | 154 | 640 | soluble acid invertase [Saccharum hybrid cultivar Pindar] |
| GenBank | ACY78464.1 | 0 | 1 | 487 | 72 | 558 | soluble acid invertase [Sorghum bicolor] |
| GenBank | ACY78465.1 | 0 | 1 | 487 | 72 | 558 | soluble acid invertase [Sorghum bicolor] |
| GenBank | ACY78467.1 | 0 | 1 | 487 | 72 | 558 | soluble acid invertase [Sorghum bicolor] |
| RefSeq | XP_002451357.1 | 0 | 1 | 487 | 1 | 487 | hypothetical protein SORBIDRAFT_04g000620 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 1 | 482 | 80 | 544 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 3ugh_A | 0 | 1 | 482 | 80 | 544 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 3ugg_B | 0 | 1 | 482 | 80 | 544 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 3ugg_A | 0 | 1 | 482 | 80 | 544 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
| PDB | 3ugf_B | 0 | 1 | 482 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| fructan biosynthesis | RXN-1803 | EC-2.4.1.10 | levansucrase |
| fructan biosynthesis | RXN-1804 | EC-2.4.1.10 | levansucrase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EC892180 | 295 | 197 | 488 | 0 |
| GO882338 | 354 | 91 | 439 | 0 |
| FL822747 | 276 | 125 | 400 | 0 |
| EE035751 | 269 | 197 | 464 | 0 |
| FL883068 | 255 | 229 | 483 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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