| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb04g010980.1 |
| Family | GH16 |
| Protein Properties | Length: 291 Molecular Weight: 32096 Isoelectric Point: 6.4862 |
| Chromosome | Chromosome/Scaffold: 4 Start: 15639149 End: 15640522 |
| Description | xyloglucan endotransglucosylase/hydrolase 26 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 39 | 206 | 2.4e-23 |
| FTSDGNGLSMSLVSNSSGCMLRTKKQYIYGTVSTLIQLVPGNSAGTVTTYYASSVGDNHDEIDFEFLGNVSGQPYTFHTNIYTNGVGNKEIQFKPWFDPT TGYHNYTISWSPCMIVWYVDSIPIRVFRNYQESYGVAYPTSRPMYAYSSIWAAEDWATQGGRVKTDWS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 291 Download |
| MVRLRALLLV AVAAAVAGLA AANFRDDCDI PWEPQNAKFT SDGNGLSMSL VSNSSGCMLR 60 TKKQYIYGTV STLIQLVPGN SAGTVTTYYA SSVGDNHDEI DFEFLGNVSG QPYTFHTNIY 120 TNGVGNKEIQ FKPWFDPTTG YHNYTISWSP CMIVWYVDSI PIRVFRNYQE SYGVAYPTSR 180 PMYAYSSIWA AEDWATQGGR VKTDWSKAPF VANYQNIHLD VCECGGGGGG CSINCPAAAA 240 KPNNGACQLS PDQRELMQSR QRNYTIYDYC VDSKRWTNGI KPTECSLPQY * 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 3.0e-22 | 32 | 195 | 174 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 5.0e-26 | 32 | 217 | 203 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 1.0e-68 | 24 | 207 | 186 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 2.0e-100 | 43 | 290 | 254 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 4.0e-125 | 23 | 285 | 264 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAY85374.1 | 0 | 25 | 289 | 26 | 291 | hypothetical protein OsI_06752 [Oryza sativa Indica Group] |
| RefSeq | NP_001046555.1 | 0 | 25 | 289 | 26 | 291 | Os02g0280300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002438432.1 | 0 | 1 | 290 | 7 | 294 | hypothetical protein SORBIDRAFT_10g019390 [Sorghum bicolor] |
| RefSeq | XP_002451950.1 | 0 | 7 | 290 | 19 | 295 | hypothetical protein SORBIDRAFT_04g010760 [Sorghum bicolor] |
| RefSeq | XP_002451953.1 | 0 | 1 | 290 | 1 | 290 | hypothetical protein SORBIDRAFT_04g010980 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 32 | 286 | 25 | 273 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1un1_A | 0 | 32 | 286 | 25 | 273 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1umz_B | 0 | 32 | 286 | 25 | 273 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 32 | 286 | 25 | 273 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 32 | 275 | 28 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
