| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb04g022700.1 |
| Family | GT8 |
| Protein Properties | Length: 645 Molecular Weight: 71995.5 Isoelectric Point: 7.3402 |
| Chromosome | Chromosome/Scaffold: 4 Start: 52291599 End: 52294172 |
| Description | plant glycogenin-like starch initiation protein 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 319 | 528 | 7.9e-35 |
| YATILHSEQLYACGAITAARSIRMAGSGRDMVALVDETISARHRGALEAAGWVVRTIRRIRNPRASRDAYNEWNYSKFWLWTLTEYERVIFLDADLLVQR PMEPLFAMPEVSATGNHGAYFNSGVMVVEPCNCTFRLLADHVGDIESYNGGDQGYLNEVFAWWHRLPSHANYMKHFWGGDTAERAAAKRRVLAADPPVAL AVHFVGLKPW | |||
| Full Sequence |
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| Protein Sequence Length: 645 Download |
| MKSSAITKGS SLSRKDCIGS YYAKDAKYRP FSALLPEGWS GKVLYVKLVL VLLMCGSFMG 60 LLNSPSIHLA DEHHHHTTQP EAASKSSWMS HPDAANSGYA SSLRIDWSQI ETAAEHFAPA 120 AADGGGTSRT RTRVALLNFD DGEVEEWKAR MPHTDASAVR LDHVGSDVTW EHLYPEWIDE 180 EERYGAPACP DLPEPSVESE EARYDVVAVK LPCGRAASWS KDVARLHLQL AAARLAARAR 240 HGGGPAAAHV LVVSRCFPMP NLFRCRDEVA RDGDVWLYRP DVSDLRRKLE LPVGSCKLAM 300 PFRALGEPYV SAAPQREAYA TILHSEQLYA CGAITAARSI RMAGSGRDMV ALVDETISAR 360 HRGALEAAGW VVRTIRRIRN PRASRDAYNE WNYSKFWLWT LTEYERVIFL DADLLVQRPM 420 EPLFAMPEVS ATGNHGAYFN SGVMVVEPCN CTFRLLADHV GDIESYNGGD QGYLNEVFAW 480 WHRLPSHANY MKHFWGGDTA ERAAAKRRVL AADPPVALAV HFVGLKPWFC FRDYDCNWNV 540 PALRQFASDE AHARWWKVHD AMPPRLQGFC LLDERQKALL RWDVARAREA NFSDGHWSAR 600 IADPRRSICA GGGDAEACRE REIAGRRVEG NRITTSYAKL IDSF* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04194 | GT8_A4GalT_like | 8.0e-11 | 401 | 528 | 162 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| PLN00176 | PLN00176 | 6.0e-11 | 306 | 427 | 125 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 1.0e-11 | 329 | 460 | 141 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 8.0e-29 | 329 | 528 | 243 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 9.0e-63 | 317 | 562 | 256 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD15458.1 | 0 | 10 | 644 | 9 | 654 | lycogenin glucosyltransferase (glycogenin)-like protein [Oryza sativa Japonica Group] |
| GenBank | EEE57188.1 | 0 | 10 | 644 | 9 | 596 | hypothetical protein OsJ_07131 [Oryza sativa Japonica Group] |
| RefSeq | NP_001044991.1 | 0 | 31 | 609 | 37 | 598 | Os01g0880200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001047126.1 | 0 | 5 | 644 | 11 | 661 | Os02g0556000 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002453984.1 | 0 | 1 | 644 | 1 | 644 | hypothetical protein SORBIDRAFT_04g022700 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1zcv_A | 2e-16 | 317 | 574 | 24 | 289 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3usr_A | 5e-16 | 317 | 574 | 24 | 289 | A Chain A, Structure Of Y194f Glycogenin Mutant Truncated At Residue 270 |
| PDB | 3v8z_A | 5e-16 | 317 | 574 | 24 | 289 | A Chain A, Structure Of Y194f Glycogenin Mutant Truncated At Residue 270 |
| PDB | 3v8y_A | 5e-16 | 317 | 574 | 24 | 289 | A Chain A, Structure Of Apo-Glycogenin Truncated At Residue 270 |
| PDB | 1zct_B | 5e-16 | 317 | 574 | 24 | 289 | A Chain A, Structure Of Glycogenin Truncated At Residue 270 In A Complex With Udp |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE182773 | 259 | 250 | 508 | 0 |
| EC876284 | 248 | 356 | 603 | 0 |
| EE155997 | 212 | 250 | 461 | 0 |
| CO253323 | 298 | 275 | 572 | 0 |
| DY367850 | 280 | 293 | 572 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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