| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb06g015940.1 |
| Family | GH16 |
| Protein Properties | Length: 288 Molecular Weight: 31941.6 Isoelectric Point: 5.6513 |
| Chromosome | Chromosome/Scaffold: 6 Start: 44131262 End: 44132501 |
| Description | xyloglucan endotransglucosylase/hydrolase 25 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 34 | 206 | 2.1e-26 |
| TDGNVRADYNAEGQQVTSLILNQQSGGAFRSRQRYLYGEFSIQMKLIPGNSAGTVTSFYLTSGDGDGHDEIDMEFMGNSSGDPVVLNTNVWANGDGKKEH QFYLWFDPAADFHTYTIIWNDKNVIFKVDDLFIRSFKRYRDLPYPGGKPMSVHATLWDGSYWATQQGKVKVDW | |||
| Full Sequence |
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| Protein Sequence Length: 288 Download |
| MARRAVSVAV VALALLAAAT AASADSWLYE KFTTDGNVRA DYNAEGQQVT SLILNQQSGG 60 AFRSRQRYLY GEFSIQMKLI PGNSAGTVTS FYLTSGDGDG HDEIDMEFMG NSSGDPVVLN 120 TNVWANGDGK KEHQFYLWFD PAADFHTYTI IWNDKNVIFK VDDLFIRSFK RYRDLPYPGG 180 KPMSVHATLW DGSYWATQQG KVKVDWSNSP FVVSYRGYTA DACVPTGGGD VGAPLSCPAG 240 TDRWMNRQLD AAEWGTVAWA KKNYMHYNYC DDGWRFPQGF PAECSRN* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam06955 | XET_C | 6.0e-17 | 237 | 284 | 51 | + Xyloglucan endo-transglycosylase (XET) C-terminus. This family represents the C-terminus (approximately 60 residues) of plant xyloglucan endo-transglycosylase (XET). Xyloglucan is the predominant hemicellulose in the cell walls of most dicotyledons. With cellulose, it forms a network that strengthens the cell wall. XET catalyzes the splitting of xyloglucan chains and the linking of the newly generated reducing end to the non-reducing end of another xyloglucan chain, thereby loosening the cell wall. Note that all family members contain the pfam00722 domain. | ||
| cd02175 | GH16_lichenase | 2.0e-20 | 58 | 163 | 108 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| pfam00722 | Glyco_hydro_16 | 2.0e-60 | 46 | 207 | 163 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 5.0e-70 | 52 | 286 | 240 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 2.0e-120 | 29 | 284 | 258 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACG39534.1 | 0 | 1 | 287 | 8 | 290 | xyloglucan endotransglycosylase/hydrolase protein 8 precursor [Zea mays] |
| GenBank | ACS13757.1 | 0 | 25 | 286 | 23 | 282 | xyloglucan xyloglucosyl transferase [Hordeum vulgare] |
| RefSeq | NP_001151500.1 | 0 | 1 | 287 | 6 | 286 | LOC100285134 [Zea mays] |
| RefSeq | XP_002446425.1 | 0 | 23 | 287 | 20 | 277 | hypothetical protein SORBIDRAFT_06g015930 [Sorghum bicolor] |
| RefSeq | XP_002447796.1 | 0 | 1 | 287 | 1 | 287 | hypothetical protein SORBIDRAFT_06g015940 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 46 | 287 | 35 | 275 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1un1_A | 0 | 46 | 287 | 35 | 275 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1umz_B | 0 | 46 | 287 | 35 | 275 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 46 | 287 | 35 | 275 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 47 | 275 | 37 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| Transmembrane Domains | ||||
|---|---|---|---|---|
| Start | End | |||
| 7 | 29 | |||
| Signal Peptide | ||||
|---|---|---|---|---|
| Cleavage Site | ||||
| 24 | ||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CO456605 | 257 | 25 | 281 | 0 |
| EE174431 | 257 | 25 | 281 | 0 |
| CA151818 | 245 | 25 | 269 | 0 |
| EC902220 | 245 | 25 | 269 | 0 |
| DR963014 | 245 | 25 | 269 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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