| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb06g023760.1 |
| Family | GH32 |
| Protein Properties | Length: 680 Molecular Weight: 73153.2 Isoelectric Point: 4.8102 |
| Chromosome | Chromosome/Scaffold: 6 Start: 52800327 End: 52804695 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 142 | 470 | 0 |
| HFQPPKNWMNDPNGPLYHKGWYHLFYQWNPDSAVWGNITWGHAVSRDLLHWLHLPLAMVPDHPYDANGVWSGSATRLPDGRIVMLYTGSTAESVQVQNLA EPADASDPLLREWVKSDANPVLVPPPGIGATDFRDPTTAWRAANDDTNSKQAWRVAIGSKDRDHAGLALVYRTEDFVRYDPVPALMHVVPGTGMWECVDF YPVAVAANNGDGLETSVPPGPGVKHVVKASLDDDKHDYYAIGTYDPATDTWTPDDAENDVGIGLRYDYGKYYASKTFYDPVLRRRVLWGWVGETDSERAD ILKGWASVQSIPRTVLLDTKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 680 Download |
| MIPAVADPAT LDDVGASTPL LPETDPRGRA GAGQKRPPPP AATTTVLPAV VSAVLLLVLV 60 AVTVLASQHV DDGQAGGVAV VVPAGGSGGG GHAVVQEVAA SRGVAEGVSE KSTAPLLGAG 120 AGAVEDFSWT NAMLAWQRTA FHFQPPKNWM NDPNGPLYHK GWYHLFYQWN PDSAVWGNIT 180 WGHAVSRDLL HWLHLPLAMV PDHPYDANGV WSGSATRLPD GRIVMLYTGS TAESVQVQNL 240 AEPADASDPL LREWVKSDAN PVLVPPPGIG ATDFRDPTTA WRAANDDTNS KQAWRVAIGS 300 KDRDHAGLAL VYRTEDFVRY DPVPALMHVV PGTGMWECVD FYPVAVAANN GDGLETSVPP 360 GPGVKHVVKA SLDDDKHDYY AIGTYDPATD TWTPDDAEND VGIGLRYDYG KYYASKTFYD 420 PVLRRRVLWG WVGETDSERA DILKGWASVQ SIPRTVLLDT KTGSNLLQWP VVEVENLRMS 480 GKRFDDVALH RGSVVPLDVG KATQLDIEAV FEVDAAAVEG VTEADVTFNC STSAGAAGRG 540 LLGPFGLLVL ADEDLSEQTA VYFYLVKGTD GSLQTFFCQD ELRASKANDL VKRVYGSLVP 600 VLDGENLSVR ILVDHSIVES FAQGGRTCIT SRVYPTRAIY DSARVFLFNN ATDVHVKAKS 660 VKIWQLNSAY IRPYEASSL* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-51 | 138 | 635 | 514 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 5.0e-68 | 138 | 668 | 539 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-88 | 148 | 473 | 331 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 6.0e-140 | 142 | 470 | 336 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 8.0e-177 | 142 | 625 | 493 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAH67112.1 | 0 | 16 | 674 | 4 | 647 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
| GenBank | EEC77707.1 | 0 | 1 | 674 | 1 | 671 | hypothetical protein OsI_16783 [Oryza sativa Indica Group] |
| RefSeq | NP_001053422.1 | 0 | 1 | 674 | 1 | 654 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | P49175 | 0 | 1 | 678 | 1 | 667 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
| RefSeq | XP_002446857.1 | 0 | 1 | 679 | 1 | 679 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 127 | 674 | 8 | 544 | A Chain A, Engineered Thermoplasma Acidophilum F3 Factor Mimics Human Aminopeptidase N (Apn) As A Target For Anticancer Drug Development |
| PDB | 3ugh_A | 0 | 127 | 674 | 8 | 544 | A Chain A, Engineered Thermoplasma Acidophilum F3 Factor Mimics Human Aminopeptidase N (Apn) As A Target For Anticancer Drug Development |
| PDB | 3ugg_B | 0 | 127 | 674 | 8 | 544 | A Chain A, Engineered Thermoplasma Acidophilum F3 Factor Mimics Human Aminopeptidase N (Apn) As A Target For Anticancer Drug Development |
| PDB | 3ugg_A | 0 | 127 | 674 | 8 | 544 | A Chain A, Engineered Thermoplasma Acidophilum F3 Factor Mimics Human Aminopeptidase N (Apn) As A Target For Anticancer Drug Development |
| PDB | 3ugf_B | 0 | 127 | 674 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE179945 | 250 | 97 | 345 | 0 |
| EE291957 | 250 | 97 | 345 | 0 |
| EC879408 | 250 | 97 | 345 | 0 |
| EC888475 | 246 | 101 | 345 | 0 |
| EE032343 | 246 | 101 | 345 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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