| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb06g031910.1 |
| Family | GH32 |
| Protein Properties | Length: 588 Molecular Weight: 64293.2 Isoelectric Point: 5.246 |
| Chromosome | Chromosome/Scaffold: 6 Start: 60225082 End: 60227279 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 43 | 360 | 0 |
| HFQPAKNWQNGPVYYNGMYHLFYQYNPHGALWDVGNLSWGHSVSGDLVNWAALGNALDPTAPFDANGCASGSVTILPDGTPGILYSGIDTDRRQVQNIAF PKNPRDPLLREWAKPAYNPVVPLPADVSANDFRDPTTAWLGRDGLWRFAISAVADGVGATLVYRSADFLRWERRATPLHASRDAVMAECPDLFPVATRGG AEEGLDTSASGKGVRHVLKVSMPDTLEDYYAVGTYDDGADTFTPDEDGDYRSWRRIDRGHLYASKTFFDARRSRRVLWAWVNESDSEADDVARGWSGLQS FPRALWLDGGGKQLVQWP | |||
| Full Sequence |
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| Protein Sequence Length: 588 Download |
| MAALLLAIRV ITVFLICLLL PTSSSSSSIC IARTQDHVRT AFHFQPAKNW QNGPVYYNGM 60 YHLFYQYNPH GALWDVGNLS WGHSVSGDLV NWAALGNALD PTAPFDANGC ASGSVTILPD 120 GTPGILYSGI DTDRRQVQNI AFPKNPRDPL LREWAKPAYN PVVPLPADVS ANDFRDPTTA 180 WLGRDGLWRF AISAVADGVG ATLVYRSADF LRWERRATPL HASRDAVMAE CPDLFPVATR 240 GGAEEGLDTS ASGKGVRHVL KVSMPDTLED YYAVGTYDDG ADTFTPDEDG DYRSWRRIDR 300 GHLYASKTFF DARRSRRVLW AWVNESDSEA DDVARGWSGL QSFPRALWLD GGGKQLVQWP 360 VEEIETLRTR RAPPLEGAEL EPAGGLREVT GIRSSQADVD VVFEIPSLGR AEGLDPSRLA 420 DPDALCREKG ASVRGGVGPF GLLVMASGDL HEHTAVFFRV FRLLHEYAVL MCTDLSRSYT 480 KADVYKPTHG GFINVDIEKD MSISLRTLID HSIVESFGGG GRTCMTARVY PEHVVTGSSH 540 LYVFNNGSDA VKVSKLEAWE LASASVNVDD DGGLVGSSVN MCHSEMY* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-40 | 35 | 369 | 368 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-56 | 34 | 539 | 529 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-81 | 52 | 363 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-125 | 43 | 360 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 3.0e-154 | 43 | 521 | 492 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE03580.1 | 0 | 1 | 586 | 3 | 592 | OSJNBa0087O24.3 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH65975.1 | 0 | 1 | 586 | 3 | 592 | H1005F08.4 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001054172.1 | 0 | 1 | 572 | 3 | 579 | Os04g0664800 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q56UD0 | 0 | 1 | 586 | 3 | 595 | INV6_ORYSJ RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 6; AltName: Full=Sucrose hydrolase 6; AltName: Full=Invertase 6; AltName: Full=Cell wall beta-fructosidase 6; AltName: Full=OsCIN6; Flags: Precursor |
| RefSeq | XP_002448711.1 | 0 | 21 | 587 | 21 | 587 | hypothetical protein SORBIDRAFT_06g031910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qqw_A | 0 | 39 | 567 | 5 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2ac1_A | 0 | 39 | 567 | 9 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 39 | 567 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 39 | 567 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 39 | 567 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
