y
Basic Information | |
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Species | Sorghum bicolor |
Cazyme ID | Sb06g031930.1 |
Family | GH32 |
Protein Properties | Length: 600 Molecular Weight: 65384.1 Isoelectric Point: 5.0471 |
Chromosome | Chromosome/Scaffold: 6 Start: 60232705 End: 60235701 |
Description | beta-fructofuranosidase 5 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 47 | 374 | 0 |
HFQPAKNWQNDPNGPMYYNGMYHFFYQYNPHGALWDIGNLSWGHSVSGDLVNWAALDTALDPTSPFDANGCWSGSATILPGGTPAILYTGIDASGEQVQN VAFPRNPADPLLREWDKPSYNPVIPLPADVPGDKFRDPSTAWLGRDGLWRIVVSAEVRGVASTLVYRSPDFLRWERAATAPLHASRAAGMVECPDLFPVK EESGGDDDGEGLDASASGDGVRHVLKLSVMDTLQDYYMVGRYDAAADAFVPAEPERGDDVRAWRRLDYGHVYASKSFFDARHSRRVLWAWANESDSQADD VAKGWSGVQTFPRKLWLDEDGKQLRQWP |
Full Sequence |
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Protein Sequence Length: 600 Download |
MAGLPLSVVA FHLCLLASSS SSSALRPATT ASESDGGGSR AGRTAYHFQP AKNWQNDPNG 60 PMYYNGMYHF FYQYNPHGAL WDIGNLSWGH SVSGDLVNWA ALDTALDPTS PFDANGCWSG 120 SATILPGGTP AILYTGIDAS GEQVQNVAFP RNPADPLLRE WDKPSYNPVI PLPADVPGDK 180 FRDPSTAWLG RDGLWRIVVS AEVRGVASTL VYRSPDFLRW ERAATAPLHA SRAAGMVECP 240 DLFPVKEESG GDDDGEGLDA SASGDGVRHV LKLSVMDTLQ DYYMVGRYDA AADAFVPAEP 300 ERGDDVRAWR RLDYGHVYAS KSFFDARHSR RVLWAWANES DSQADDVAKG WSGVQTFPRK 360 LWLDEDGKQL RQWPVEEIET LRRKRVGLRR GTVLSAGGMN EIVGVAGSQA DVEVEFKVPS 420 LAEAEALDAN RLLDPQKLCG EKGASVPGGV GPFGLIVLAS GDLREHTTVF FRVFRHQGRY 480 RLLMCTDLTR STTRAGVYKP PYGGFVDVDV EEHETIKLRT LIDHSMLESF GAEGRICITA 540 RVYPEHAETS NSHLFVFNNG TGKVEVPKLE AWELAAATVN AVGDAGLIVS QSKDESESY* 600 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-45 | 42 | 544 | 525 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-65 | 43 | 566 | 540 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-89 | 53 | 377 | 336 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-131 | 47 | 374 | 338 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 9.0e-162 | 47 | 534 | 498 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
Swiss-Prot | A2YZ01 | 0 | 3 | 582 | 5 | 575 | INV7_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7; AltName: Full=Sucrose hydrolase 7; AltName: Full=Invertase 7; AltName: Full=Cell wall beta-fructosidase 7; AltName: Full=OsCIN7; Flags: Precursor |
GenBank | AAD38399.1 | 0 | 3 | 582 | 5 | 575 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
GenBank | EEC84182.1 | 0 | 3 | 582 | 5 | 574 | hypothetical protein OsI_30564 [Oryza sativa Indica Group] |
RefSeq | NP_001104899.1 | 0 | 1 | 596 | 1 | 597 | invertase cell wall4 [Zea mays] |
RefSeq | XP_002448713.1 | 0 | 1 | 599 | 1 | 599 | hypothetical protein SORBIDRAFT_06g031930 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 43 | 577 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 2ade_A | 0 | 43 | 577 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 2add_A | 0 | 43 | 577 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 1st8_A | 0 | 43 | 577 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 43 | 577 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |