| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb07g007010.1 |
| Family | GH16 |
| Protein Properties | Length: 283 Molecular Weight: 32384.1 Isoelectric Point: 4.3614 |
| Chromosome | Chromosome/Scaffold: 7 Start: 10770828 End: 10771917 |
| Description | xyloglucan endotransglucosylase/hydrolase 15 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 45 | 207 | 9.2e-29 |
| TLALGLDHTSGSGFSSKDAYLYGRFDMDIKLVSNNSAGTVATFYLTPDDVPWEYHDEVDMEFLGNATGEPYTLHTNVYLNGVGSREQQFHLWFDPAEDFH TYSIEWNPKYIIFLVDGTPIRVYKNDRARGVPFPTWQRMRVQGSLWNADEWATQGGRVKTDWT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 283 Download |
| MVVMAFITLA MAYSTAVVAL LYDDIEISWG QDCYFYMDGD TDTDTLALGL DHTSGSGFSS 60 KDAYLYGRFD MDIKLVSNNS AGTVATFYLT PDDVPWEYHD EVDMEFLGNA TGEPYTLHTN 120 VYLNGVGSRE QQFHLWFDPA EDFHTYSIEW NPKYIIFLVD GTPIRVYKND RARGVPFPTW 180 QRMRVQGSLW NADEWATQGG RVKTDWTQAP FYAYYRNFRV TPCVPSPGVA WCGDEPAQST 240 WFDRRLDSAA LRKVQEENMV YDYCEDQKRF KDKGLPVECT KA* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-24 | 52 | 221 | 176 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-27 | 52 | 222 | 184 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-66 | 54 | 207 | 155 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 5.0e-86 | 1 | 282 | 291 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 1.0e-121 | 16 | 279 | 270 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAZ06117.1 | 0 | 23 | 280 | 40 | 298 | hypothetical protein OsI_28354 [Oryza sativa Indica Group] |
| GenBank | EAZ06118.1 | 0 | 23 | 280 | 36 | 294 | hypothetical protein OsI_28355 [Oryza sativa Indica Group] |
| GenBank | EAZ41980.1 | 0 | 21 | 280 | 1 | 261 | hypothetical protein OsJ_26529 [Oryza sativa Japonica Group] |
| RefSeq | NP_001061321.1 | 0 | 23 | 280 | 41 | 299 | Os08g0237800 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002444081.1 | 0 | 23 | 282 | 23 | 282 | hypothetical protein SORBIDRAFT_07g007010 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 15 | 281 | 3 | 274 | A Chain A, Barley 1,3-1,4-Beta-Glucanase In Monoclinic Space Group |
| PDB | 1un1_A | 0 | 15 | 281 | 3 | 274 | A Chain A, Barley 1,3-1,4-Beta-Glucanase In Monoclinic Space Group |
| PDB | 1umz_B | 0 | 15 | 281 | 3 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 15 | 281 | 3 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 42 | 279 | 36 | 267 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
