| Basic Information | |
|---|---|
| Species | Sorghum bicolor |
| Cazyme ID | Sb09g025790.1 |
| Family | GT20 |
| Protein Properties | Length: 978 Molecular Weight: 108667 Isoelectric Point: 6.7424 |
| Chromosome | Chromosome/Scaffold: 9 Start: 55191533 End: 55199672 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 126 | 594 | 0 |
| MKQRLLVVANRLPVSANRRGEDHWSLEISAGGLVSALLGVKDVDAKWIGWAGVNVPDEVGQRALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRNFESQFDAYKRANQMFADVVYQHYQEGDVIWCHDYHLMFLPKCLKDHDINMKVGWFLHTPFPSSEIYRTLPSRLELLRSVLCADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFKRALELPAVKRHISELTQRFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NPDWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEYVACQGSKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVADSIRHALTMPSDEREKRHRHNYAHVTTHTAQDWAETFVFELN | |||
| Full Sequence |
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| Protein Sequence Length: 978 Download |
| MSSDAAGGQR SISNSTRGDA AAAMPTSSPF VVGDSSGGAG SPIRVDRMVR EHGRRYDIFA 60 SDAMDTDGAE PASASAGPFA VDGVQSPGRV SPANMEDAGG AAAGHAARPP LAGSRSGFRR 120 LGLRGMKQRL LVVANRLPVS ANRRGEDHWS LEISAGGLVS ALLGVKDVDA KWIGWAGVNV 180 PDEVGQRALT KALAEKRCIP VFLDEEIVHQ YYNGYCNNIL WPLFHYLGLP QEDRLATTRN 240 FESQFDAYKR ANQMFADVVY QHYQEGDVIW CHDYHLMFLP KCLKDHDINM KVGWFLHTPF 300 PSSEIYRTLP SRLELLRSVL CADLVGFHTY DYARHFVSAC TRILGLEGTP EGVEDQGRLT 360 RVAAFPIGID SDRFKRALEL PAVKRHISEL TQRFAGRKVM LGVDRLDMIK GIPQKILAFE 420 KFLEENPDWN DKVVLLQIAV PTRTDVPEYQ KLTSQVHEIV GRINGRFGTL TAVPIHHLDR 480 SLDFHALCAL YAVTDVALVT SLRDGMNLVS YEYVACQGSK KGVLILSEFA GAAQSLGAGA 540 ILVNPWNITE VADSIRHALT MPSDEREKRH RHNYAHVTTH TAQDWAETFV FELNDTVAEA 600 LLRTRQVPPG LPSQTAIQQY LRSKNRLLIL GFNSTLTEPV ESSGRRGGDQ IKEMELKLHP 660 DLKGPLRALC EDERTTVIVL SGSDRSVLDE NFGEFKMWLA AEHGMFLRPT YGEWMTTMPE 720 HLNMDWVDSV KHVFEYFTER TPRSHFEHRE TSFVWNYKYA DVEFGRLQAR DMLQHLWTGP 780 ISNAAVDVVQ GSRSVEVRSV GVTKGAAIDR ILGEIVHSEN MVTPIDYVLC IGHFLGKDED 840 IYVFFDPEYP SESKIKPEGG SASLDRRPNG RPPSNGRSNS RNPQSRTQKA QQAQAASERS 900 SSSSHSSASS NHDWREGSSV LDLKGENYFS CAVGRKRSNA RYLLSSSEEV VSFLKELATA 960 TAGFQSSCAD YMFLDRQ* 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 33 | 965 | 938 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 127 | 970 | 844 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 128 | 595 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| TIGR02400 | trehalose_OtsA | 0 | 129 | 595 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 129 | 832 | 709 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABF70084.1 | 0 | 20 | 962 | 4 | 961 | trehalose-6-phosphate synthase, putative [Musa balbisiana] |
| GenBank | ACI16353.1 | 0 | 126 | 977 | 1 | 859 | trehalose-6-P synthase [Triticum aestivum] |
| GenBank | EAY98715.1 | 0 | 126 | 977 | 1 | 855 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
| RefSeq | NP_001123593.2 | 0 | 24 | 972 | 1 | 935 | trehalose-6-phosphate synthase [Zea mays] |
| RefSeq | XP_002440086.1 | 0 | 1 | 977 | 1 | 977 | hypothetical protein SORBIDRAFT_09g025790 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 129 | 597 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 129 | 597 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 129 | 597 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 129 | 597 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 129 | 597 | 3 | 455 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB651133 | 281 | 155 | 435 | 0 |
| DW069910 | 288 | 215 | 502 | 0 |
| EH189441 | 291 | 497 | 787 | 0 |
| CB629539 | 271 | 307 | 577 | 0 |
| CV711307 | 293 | 127 | 419 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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