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Basic Information | |
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Species | Sorghum bicolor |
Cazyme ID | Sb10g027110.1 |
Family | GH31 |
Protein Properties | Length: 897 Molecular Weight: 98334.9 Isoelectric Point: 9.2819 |
Chromosome | Chromosome/Scaffold: 10 Start: 56788632 End: 56793054 |
Description | Glycosyl hydrolases family 31 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 280 | 747 | 0 |
FYFFAGPAPLDVVDQYTQLIGRPAPMPYWSFGFHQCRYGYKNLADLEGVVAGYAKARIPLEVMWTDIDYMDAFKDFTLDPVNFPAVPMRQFVDRLHRNGQ KYVVIIDPGINVNQTYGTFVRGMQQDVFLKRNGTNYLGKVWPGYVYFPDFLNPRAAEFWAREIALFRRTLLPVDGLWIDMNEVSNFVDPAPLNALDDPPY RINNSGVRRPINNKTTPASAVHYGGVREYDAHNLYGFLEARATHGALLADTGRRPFVLSRSTFVGSGRYTAHWTGDNAATWDDLRYSINTMLSFGLFGIP MVGADICGFGGNTTEELCSRWIQLGAFYPFARDHSAIGTIRRELYLWEVVARSARKALGLRYRLLPYLYTLMHEAHTTGAPIARPLFFSYPKDAATYDVD RQFLLGRGVLVSPVLEPGATTVDAYFPAGRWFSLYDYTLAVASATGKRVTLPAPADTVNVHVAGGNIL |
Full Sequence |
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Protein Sequence Length: 897 Download |
MAWPWPPPPP RSTAHRRLLF LVLLLSLLAA TPWPSAAAQV AGYRVVSVAR ARARGGQLSA 60 RLELAGGAGG KPELGPDVQR LSLTASLETD SRLHVRITNA DHPRWEVPQS VIPREAPRQI 120 TLESSTGAAS PHSRVLSAAT SDLTFTLHAS PFRFTVSRRS SGDVLFDTSA ALVFKDRYLE 180 LTTALPADVR ASSLYGLGEH TKRTFRLQRN DTFTLWNADI PASTVDVNLY GSHPFYLDVR 240 HPASGGGAAH GVLLLNSNGM DVEYGGSYLT YKVIGGVLDF YFFAGPAPLD VVDQYTQLIG 300 RPAPMPYWSF GFHQCRYGYK NLADLEGVVA GYAKARIPLE VMWTDIDYMD AFKDFTLDPV 360 NFPAVPMRQF VDRLHRNGQK YVVIIDPGIN VNQTYGTFVR GMQQDVFLKR NGTNYLGKVW 420 PGYVYFPDFL NPRAAEFWAR EIALFRRTLL PVDGLWIDMN EVSNFVDPAP LNALDDPPYR 480 INNSGVRRPI NNKTTPASAV HYGGVREYDA HNLYGFLEAR ATHGALLADT GRRPFVLSRS 540 TFVGSGRYTA HWTGDNAATW DDLRYSINTM LSFGLFGIPM VGADICGFGG NTTEELCSRW 600 IQLGAFYPFA RDHSAIGTIR RELYLWEVVA RSARKALGLR YRLLPYLYTL MHEAHTTGAP 660 IARPLFFSYP KDAATYDVDR QFLLGRGVLV SPVLEPGATT VDAYFPAGRW FSLYDYTLAV 720 ASATGKRVTL PAPADTVNVH VAGGNILPLQ LPAPTTSRAR RTAFHLLVAL GEDGAASGEL 780 FLDDGESPEM AGSRGKWSLV RFSCAAGHDG VTVRSHVVRD AYGPTRKLAI GKVIFLGLHS 840 PAAPQREFSV YVNGVKTANS IGGALGYQRN GAFGAAQVEG LSLAVGKEFE LKVVML* 900 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06600 | GH31_MGAM-like | 7.0e-104 | 300 | 658 | 362 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
cd06604 | GH31_glucosidase_II_MalA | 7.0e-119 | 300 | 658 | 365 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 4.0e-137 | 140 | 787 | 665 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 281 | 747 | 471 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 0 | 300 | 676 | 382 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | EAZ02070.1 | 0 | 77 | 895 | 70 | 884 | hypothetical protein OsI_24149 [Oryza sativa Indica Group] |
GenBank | EEC81171.1 | 0 | 41 | 895 | 33 | 895 | hypothetical protein OsI_24144 [Oryza sativa Indica Group] |
RefSeq | NP_001058347.1 | 0 | 41 | 895 | 33 | 883 | Os06g0675700 [Oryza sativa (japonica cultivar-group)] |
RefSeq | NP_001058353.1 | 0 | 77 | 895 | 70 | 884 | Os06g0676700 [Oryza sativa (japonica cultivar-group)] |
RefSeq | XP_002438844.1 | 0 | 11 | 896 | 11 | 896 | hypothetical protein SORBIDRAFT_10g027110 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 42 | 893 | 44 | 906 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
PDB | 3w37_A | 0 | 42 | 893 | 44 | 906 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
PDB | 3l4z_A | 0 | 72 | 892 | 76 | 867 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
PDB | 3l4y_A | 0 | 72 | 892 | 76 | 867 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
PDB | 3l4x_A | 0 | 72 | 892 | 76 | 867 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |