| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si000803m |
| Family | GH32 |
| Protein Properties | Length: 584 Molecular Weight: 65819.4 Isoelectric Point: 6.3033 |
| Chromosome | Chromosome/Scaffold: 5 Start: 46997354 End: 47000848 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 60 | 374 | 0 |
| HFQPPKNWINGPMYYNGIYHQFYQYNPNGSLWGNIVWGHSVSTDLINWIRLEPAIERTIPSDINGCWTGSATILKGNRPAIIYTGADTEKRQVQNIVFPK NVSDPYLREWIKPDNNPLIQPIGQGLNSGQFRDPTTGWIGPDGLWRIAVGAELNGYSAALLYKSEDFVNWTRVDHSLYSSNASTMWECPDFFAVLPGNNS GLDLSAAIPNGAKHVLKMSLDNCDKYMVGVYDLKSDTFVPDTILDDRRLWSRIDYGNYYASKSFFDSKRGRRIIWGWTNETDSSLDDVAKGWAGIHAIPR TIWLDSNSKQLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 584 Download |
| MALVLRPWAF LLFLVLVSYD ESGLQSRRNG VAQATQRVFL YPQSPKVSSI VSTKYRTAYH 60 FQPPKNWING PMYYNGIYHQ FYQYNPNGSL WGNIVWGHSV STDLINWIRL EPAIERTIPS 120 DINGCWTGSA TILKGNRPAI IYTGADTEKR QVQNIVFPKN VSDPYLREWI KPDNNPLIQP 180 IGQGLNSGQF RDPTTGWIGP DGLWRIAVGA ELNGYSAALL YKSEDFVNWT RVDHSLYSSN 240 ASTMWECPDF FAVLPGNNSG LDLSAAIPNG AKHVLKMSLD NCDKYMVGVY DLKSDTFVPD 300 TILDDRRLWS RIDYGNYYAS KSFFDSKRGR RIIWGWTNET DSSLDDVAKG WAGIHAIPRT 360 IWLDSNSKQL LQWPVEEVES LRSEEVSHQG LELKQGDLFD IKGIDTLQAD VEIDFELTSI 420 DSADPFDPSW LLDIEKHCRE ADASVHGGVG PFGLVVLASD NMEEHTSVHF RVYKSQEKYM 480 VLMCSDLRKS SLRPELYTPA YGGFFEFDLE KESTISLRTL IDRSAVESFG GGGRLCIMAR 540 VYPVTLINGG TRMYAFNNGT STVKVPQLKA WSMRRAQVNV KKG* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-41 | 52 | 543 | 520 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 6.0e-52 | 48 | 565 | 541 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-78 | 66 | 374 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-129 | 60 | 374 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-145 | 60 | 533 | 489 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACL54520.1 | 0 | 18 | 579 | 20 | 588 | unknown [Zea mays] |
| DDBJ | BAD05180.1 | 0 | 28 | 583 | 33 | 590 | cell wall invertase [Oryza sativa Japonica Group] |
| GenBank | EEC72233.1 | 0 | 29 | 583 | 30 | 586 | hypothetical protein OsI_05346 [Oryza sativa Indica Group] |
| RefSeq | NP_001045504.1 | 0 | 28 | 583 | 33 | 590 | Os01g0966700 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002457005.1 | 0 | 1 | 583 | 3 | 570 | hypothetical protein SORBIDRAFT_03g047060 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 48 | 579 | 1 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 55 | 579 | 4 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 55 | 579 | 4 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 55 | 579 | 4 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 55 | 579 | 4 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
