| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si001018m |
| Family | AA7 |
| Protein Properties | Length: 523 Molecular Weight: 58046.4 Isoelectric Point: 7.553 |
| Chromosome | Chromosome/Scaffold: 5 Start: 46001264 End: 46003641 |
| Description | cytokinin oxidase/dehydrogenase 1 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 58 | 254 | 7.6e-27 |
| CSLLPATVLHPGSVSDIAATVRHVFSLGERSPLTIAARGHGHSLMGQSQAAGGIVVRMESLRGDRLQVHDGSMSPFVDAPGGELWINVLHETLKYGLAPK SWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGDVVTCSPEENSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFASF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 523 Download |
| MKPSLEHCFK LLLLLALGGV TMHVPDADVL SSLGALRLYG HFSFHDATAM ARDFGNRCSL 60 LPATVLHPGS VSDIAATVRH VFSLGERSPL TIAARGHGHS LMGQSQAAGG IVVRMESLRG 120 DRLQVHDGSM SPFVDAPGGE LWINVLHETL KYGLAPKSWT DYLHLTVGGT LSNAGVSGQA 180 FRHGPQVSNV NQLEIVTGRG DVVTCSPEEN SDLFYAALGG LGQFGIITRA RIALEPAPKM 240 VRWIRVLYSD FASFTEDQEM LIMAKNTFDY IEGFVIINRT GILNNWRTSF KPQDPVQASR 300 FQSDGRVLYC LELTKNFNSN DAGTMEQEVT ALLSKLRYIR STLFHTDVTY LEFLDRVHTS 360 EVKLRAQGLW EVPHPWLNLL IPRSSIHRFA KEVFGKILKD SNNGPILLYP VNKSKWDNRT 420 SVVIPDEEIF YLVGFLSSAP SLSGHGSVAH AMNLNNQIVE FCEEADIGMK QYLAPYTTQQ 480 QWKAHFGARW ETFERRKHTY DPLAILAPGQ RIFPKASLPL SL* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 1.0e-7 | 166 | 237 | 72 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 8.0e-21 | 62 | 206 | 146 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 8.0e-26 | 61 | 510 | 457 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 3.0e-164 | 238 | 513 | 280 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 2 | 520 | 529 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE55201.1 | 0 | 1 | 522 | 1 | 525 | cytokinin oxidase 3 [Zea mays] |
| RefSeq | NP_001045353.1 | 0 | 1 | 522 | 5 | 529 | Os01g0940000 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001105163.1 | 0 | 1 | 522 | 1 | 525 | cytokinin oxidase 3 [Zea mays] |
| RefSeq | NP_001105526.1 | 0 | 3 | 522 | 4 | 519 | cytokinin oxidase 2 [Zea mays] |
| RefSeq | XP_002456917.1 | 0 | 3 | 522 | 4 | 523 | hypothetical protein SORBIDRAFT_03g045410 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 47 | 516 | 47 | 523 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 2exr_A | 0 | 47 | 516 | 47 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 31 | 513 | 18 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 31 | 513 | 18 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 31 | 513 | 18 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
