y
Basic Information | |
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Species | Setaria italica |
Cazyme ID | Si006461m |
Family | GH13 |
Protein Properties | Length: 437 Molecular Weight: 47997 Isoelectric Point: 4.9003 |
Chromosome | Chromosome/Scaffold: 4 Start: 39408968 End: 39410813 |
Description | alpha-amylase-like |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 42 | 347 | 1.7e-39 |
YNNLMGKVDDIAAAGVTHVWLPPPSHSVSSEGYMPGRLYDLDASKYGTAAELKSLIAAFHDKGVQAVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDW RAHMICRDDAKYSDGTGNLDTGADFPGAPDIDHLNDRVQRELTEWMLWLKSDLGFDAWRLDFARGYSAEVARVYINGTAPSFVVAEIWDKMVPAGDDGKP AYDQDPHRQALVDWVDKVGGAASPATVFDFTTKGILNAAVEGELWRLIDTEGKAPGVIGWWPAKAVTFVDNHDTGSTQAQWPFPHDKVMQGYAYILTHPG NPCIFY |
Full Sequence |
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Protein Sequence Length: 437 Download |
MGMGSRHMCC LSTLLLLLGL SSGQVLFQGF NWESSKQTGG WYNNLMGKVD DIAAAGVTHV 60 WLPPPSHSVS SEGYMPGRLY DLDASKYGTA AELKSLIAAF HDKGVQAVAD IVINHRCADY 120 KDSRGIYCIF EGGTPDSRLD WRAHMICRDD AKYSDGTGNL DTGADFPGAP DIDHLNDRVQ 180 RELTEWMLWL KSDLGFDAWR LDFARGYSAE VARVYINGTA PSFVVAEIWD KMVPAGDDGK 240 PAYDQDPHRQ ALVDWVDKVG GAASPATVFD FTTKGILNAA VEGELWRLID TEGKAPGVIG 300 WWPAKAVTFV DNHDTGSTQA QWPFPHDKVM QGYAYILTHP GNPCIFYDHF FDWGFKDEIA 360 ALVAVRKRNG ITPTSELTIL EHDGDAYVAE IDGKVIVKIG SRYDLGHLIP VGFEVATHGK 420 DYAVWEKGGS EEVTRA* 480 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd11318 | AmyAc_bac_fung_AmyA | 6.0e-32 | 25 | 368 | 430 | + Alpha amylase catalytic domain found in bacterial and fungal Alpha amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes bacterial and fungal proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02784 | PLN02784 | 3.0e-141 | 24 | 427 | 405 | + alpha-amylase | ||
PLN02361 | PLN02361 | 5.0e-149 | 24 | 427 | 408 | + alpha-amylase | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 3.0e-163 | 25 | 377 | 356 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN00196 | PLN00196 | 0 | 3 | 427 | 428 | + alpha-amylase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ADC54351.1 | 0 | 24 | 427 | 2 | 404 | alpha amylase [Hordeum vulgare subsp. spontaneum] |
GenBank | ADC54353.1 | 0 | 24 | 427 | 2 | 404 | alpha amylase [Hordeum vulgare subsp. spontaneum] |
GenBank | ADC54357.1 | 0 | 24 | 427 | 2 | 404 | alpha amylase [Hordeum vulgare subsp. spontaneum] |
GenBank | ADC54359.1 | 0 | 24 | 427 | 2 | 404 | alpha amylase [Hordeum vulgare subsp. spontaneum] |
RefSeq | XP_002462321.1 | 0 | 25 | 436 | 30 | 435 | hypothetical protein SORBIDRAFT_02g023790 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1rpk_A | 0 | 24 | 427 | 2 | 404 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 1p6w_A | 0 | 24 | 427 | 2 | 404 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 1ht6_A | 0 | 24 | 427 | 2 | 404 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 3bsg_A | 0 | 24 | 430 | 2 | 407 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 2qpu_C | 0 | 24 | 427 | 2 | 404 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |