| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si008391m |
| Family | AA7 |
| Protein Properties | Length: 540 Molecular Weight: 59050.8 Isoelectric Point: 5.5732 |
| Chromosome | Chromosome/Scaffold: 4 Start: 35638666 End: 35640532 |
| Description | cytokinin oxidase 5 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 60 | 256 | 7.3e-33 |
| RIVEAIPNGVFHPTSPADIAALIRLSISQSKPFTVAPRGQGHSARGQALAPGGIVIDMRSMGRGNHGYHANVSSDELWVDVGGEQLWIDVLHATLEHGLA PRIWTDYLHITVGGTLSNGGIGGQAFRHGPQISNVHELDVVTGMGEMITCSQDKNSDLFFAALGGLGQFGVITRARIALEPSPKRVLWVRIAYSDVE | |||
| Full Sequence |
|---|
| Protein Sequence Length: 540 Download |
| MPKACVATFL MMTSFLSTIN HFQMVSTVAL PDDDIFTLDI VSKIHVDHDS TAKASSDFGR 60 IVEAIPNGVF HPTSPADIAA LIRLSISQSK PFTVAPRGQG HSARGQALAP GGIVIDMRSM 120 GRGNHGYHAN VSSDELWVDV GGEQLWIDVL HATLEHGLAP RIWTDYLHIT VGGTLSNGGI 180 GGQAFRHGPQ ISNVHELDVV TGMGEMITCS QDKNSDLFFA ALGGLGQFGV ITRARIALEP 240 SPKRVLWVRI AYSDVESFTS DQELLISKRS NGSGFDYIEG QVQLNRTLTE GRRSSSFFSA 300 SELDQLAELV LRTGSNAIYY IEGAMYYNDD TASSVNQKLE RQLEELSFAP GFVFVRNVSY 360 LEFLDRIGRE EQKLRSAGVW DVPHPWLNLF VPKSRILDFD ASVFKGILRD AKPVGLILMY 420 PMNKNMWDDR MTPATPDGDV FYAVGLLRSA VAAGDLERLE GENAAVLEFC DREGVGCKQY 480 LPHHASPDGW RRHFGEKLGR VAAMKRRYDP RAILSPGQGI FPTGAAVADG DDEAGSDSL* 540 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 1.0e-6 | 56 | 240 | 186 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| COG0277 | GlcD | 3.0e-25 | 39 | 520 | 490 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 2.0e-26 | 66 | 210 | 146 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 2.0e-146 | 242 | 521 | 283 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 522 | 525 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD17196.1 | 0 | 1 | 521 | 1 | 516 | putative cytokinin dehydrogenase 1 precursor [Oryza sativa Japonica Group] |
| DDBJ | BAD61835.1 | 0 | 1 | 520 | 2 | 529 | putative cytokinin oxidase [Oryza sativa Japonica Group] |
| GenBank | EEC80843.1 | 0 | 41 | 520 | 1 | 487 | hypothetical protein OsI_23446 [Oryza sativa Indica Group] |
| RefSeq | XP_002438603.1 | 0 | 1 | 538 | 1 | 547 | hypothetical protein SORBIDRAFT_10g022590 [Sorghum bicolor] |
| RefSeq | XP_002453544.1 | 0 | 1 | 521 | 1 | 525 | hypothetical protein SORBIDRAFT_04g007730 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1w1s_A | 0 | 5 | 521 | 2 | 533 | A Chain A, Crystal Structure Of The Glycogen Synthase From A. Tumefaciens In Complex With Adp |
| PDB | 1w1r_A | 0 | 5 | 521 | 2 | 533 | A Chain A, Crystal Structure Of The Glycogen Synthase From A. Tumefaciens In Complex With Adp |
| PDB | 1w1q_A | 0 | 5 | 521 | 2 | 533 | A Chain A, Crystal Structure Of The Glycogen Synthase From A. Tumefaciens In Complex With Adp |
| PDB | 1w1o_A | 0 | 5 | 521 | 2 | 533 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 3s1e_A | 0 | 27 | 521 | 5 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
