Basic Information | |
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Species | Setaria italica |
Cazyme ID | Si009545m |
Family | GH32 |
Protein Properties | Length: 673 Molecular Weight: 72473.8 Isoelectric Point: 5.9777 |
Chromosome | Chromosome/Scaffold: 7 Start: 25927995 End: 25933106 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 137 | 463 | 0 |
HFQPPKNWMNDPNGPLYHKGWYHLFYQWNPHSAVWGNITWGHAVSRDLVHWLHLPLAMVPDHWYDANGVWSGSATRLPDGRIVMLYTGSTADSVQVQNLA EPADPSDPLLREWVKSDANPVLVPPPGIGLKDFRDPTTAWRVPNDTAWRVAIGSKDRSHAGLALVYRTEDFVRYDPDPALMHVVPGTGMWECVDFYPVAT GSGGAGENSGLETSAPPGPGVKHVVKASLDDDKHDYYAIGTYDAAADTWTPDDTVNDVGIGLRYDYGKFYASKTFYDPVLRRRVLWGWVGETDSERADIL KGWASLQSIPRTVLLDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 673 Download |
MIPPVADPAM LEGAARSPLL PATDPRRAAA PAGGAGQRRP SSSTVLPAVV SAVLLLVLAV 60 VTILASQHVD NGQRVVMPPA GGDVAAAAGR VVEVAATRGV AEGVSEKSTA PLLGGAGALR 120 DYSWTNAMLA WQRTAFHFQP PKNWMNDPNG PLYHKGWYHL FYQWNPHSAV WGNITWGHAV 180 SRDLVHWLHL PLAMVPDHWY DANGVWSGSA TRLPDGRIVM LYTGSTADSV QVQNLAEPAD 240 PSDPLLREWV KSDANPVLVP PPGIGLKDFR DPTTAWRVPN DTAWRVAIGS KDRSHAGLAL 300 VYRTEDFVRY DPDPALMHVV PGTGMWECVD FYPVATGSGG AGENSGLETS APPGPGVKHV 360 VKASLDDDKH DYYAIGTYDA AADTWTPDDT VNDVGIGLRY DYGKFYASKT FYDPVLRRRV 420 LWGWVGETDS ERADILKGWA SLQSIPRTVL LDTKTGSNLL QWPVVEVENL RMSGKSFGGV 480 ALGHGSVVPL DVGKATQLDI EAEFEVDAAA VEAVTEAEVG FNCSTSAGAA GRGMLGPFGL 540 LVLADEDRSE QTAVYFYLVK GTDGSLKTFF CQDELRGSKA NDLVKRVYGS VVPVLNGENL 600 SVRILVDHSI VESFAQGGRT CITSRVYPTR AIYDSARVFL FNNATNVHVT AKSVKIWQLN 660 SAYIRPYSAS SL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-53 | 133 | 628 | 516 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-68 | 133 | 630 | 506 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 6.0e-88 | 143 | 466 | 335 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-144 | 137 | 463 | 334 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-178 | 137 | 618 | 491 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAH67112.1 | 0 | 10 | 667 | 1 | 647 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
GenBank | EEC77707.1 | 0 | 1 | 667 | 1 | 671 | hypothetical protein OsI_16783 [Oryza sativa Indica Group] |
RefSeq | NP_001053422.1 | 0 | 1 | 667 | 1 | 654 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
Swiss-Prot | P49175 | 0 | 1 | 671 | 1 | 667 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
RefSeq | XP_002446857.1 | 0 | 1 | 672 | 1 | 679 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 122 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugh_A | 0 | 122 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_B | 0 | 122 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_A | 0 | 122 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugf_B | 0 | 122 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
fructan biosynthesis | RXN-1803 | EC-2.4.1.10 | levansucrase |
fructan biosynthesis | RXN-1804 | EC-2.4.1.10 | levansucrase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |