Basic Information | |
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Species | Setaria italica |
Cazyme ID | Si009988m |
Family | GH32 |
Protein Properties | Length: 480 Molecular Weight: 51984.4 Isoelectric Point: 4.785 |
Chromosome | Chromosome/Scaffold: 7 Start: 25927995 End: 25933106 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 2 | 270 | 0 |
VPDHWYDANGVWSGSATRLPDGRIVMLYTGSTADSVQVQNLAEPADPSDPLLREWVKSDANPVLVPPPGIGLKDFRDPTTAWRVPNDTAWRVAIGSKDRS HAGLALVYRTEDFVRYDPDPALMHVVPGTGMWECVDFYPVATGSGGAGENSGLETSAPPGPGVKHVVKASLDDDKHDYYAIGTYDAAADTWTPDDTVNDV GIGLRYDYGKFYASKTFYDPVLRRRVLWGWVGETDSERADILKGWASLQSIPRTVLLDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 480 Download |
MVPDHWYDAN GVWSGSATRL PDGRIVMLYT GSTADSVQVQ NLAEPADPSD PLLREWVKSD 60 ANPVLVPPPG IGLKDFRDPT TAWRVPNDTA WRVAIGSKDR SHAGLALVYR TEDFVRYDPD 120 PALMHVVPGT GMWECVDFYP VATGSGGAGE NSGLETSAPP GPGVKHVVKA SLDDDKHDYY 180 AIGTYDAAAD TWTPDDTVND VGIGLRYDYG KFYASKTFYD PVLRRRVLWG WVGETDSERA 240 DILKGWASLQ SIPRTVLLDT KTGSNLLQWP VVEVENLRMS GKSFGGVALG HGSVVPLDVG 300 KATQLDIEAE FEVDAAAVEA VTEAEVGFNC STSAGAAGRG MLGPFGLLVL ADEDRSEQTA 360 VYFYLVKGTD GSLKTFFCQD ELRGSKANDL VKRVYGSVVP VLNGENLSVR ILVDHSIVES 420 FAQGGRTCIT SRVYPTRAIY DSARVFLFNN ATNVHVTAKS VKIWQLNSAY IRPYSASSL* 480 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-23 | 3 | 435 | 453 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-30 | 3 | 437 | 443 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-48 | 1 | 273 | 284 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-95 | 1 | 270 | 277 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-129 | 1 | 425 | 434 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACN34201.1 | 0 | 1 | 478 | 1 | 482 | unknown [Zea mays] |
EMBL | CAH67112.1 | 0 | 1 | 474 | 175 | 647 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
RefSeq | NP_001053422.1 | 0 | 1 | 474 | 182 | 654 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
Swiss-Prot | P49175 | 0 | 1 | 478 | 186 | 667 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
RefSeq | XP_002446857.1 | 0 | 1 | 479 | 199 | 679 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 1 | 474 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugh_A | 0 | 1 | 474 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_B | 0 | 1 | 474 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_A | 0 | 1 | 474 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugf_B | 0 | 1 | 474 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FE636262 | 237 | 71 | 307 | 0 |
FL909048 | 232 | 240 | 471 | 0 |
CB651234 | 259 | 218 | 474 | 0 |
GO882338 | 345 | 91 | 431 | 0 |
GW667497 | 321 | 151 | 470 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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