| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si013532m |
| Family | AA7 |
| Protein Properties | Length: 539 Molecular Weight: 55941.4 Isoelectric Point: 8.6442 |
| Chromosome | Chromosome/Scaffold: 6 Start: 4404933 End: 4406861 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 76 | 532 | 0 |
| GKPAAVVLPSSKHGLRAAVLCARAAALAVRVRSGGHSYEGLSYTTENRVPFAVIDLAGLSRVRVDGGSATAWAEAGATLGELYHAVGTSSRTLAFPAGSC STIGLGGIVSGGGFGLLSRKHGLAADNVLDAVLIDPSGNILTRDTMGDDVFWAIRGGGGGSWGVVYAWKLRLVPVPDTITVFTASRTGPVELVAGLVHRW QFVGPNLPDDFYLSVYLPTGGGSSPDSNVSVSFTGQVLGPKRRAMAALRRSFPELGLAASELAETSWLDATAQFAGLATAADLPDRRLGSKQYSKGKSDY VRSPIPLHTMAGIVRYLATGPPEGGCVILDPYGGAMARVGSGATPFPHRAGTLYGVQYQVYWDEDAGEAAPVGWLRSLHSFMAPHVSRAPRGAYVNYLDL DLGVNDWTVAAGGSSEAAVARARASWGAAYFGDNFDRLVRAKTAVDPGNVFNNAQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 539 Download |
| MGSISIAALA LLVPLFLSLH QTTCSAVGAG DDAARNLSSC LTSHGVTTFS LPSSPTYAAL 60 LNSSIRNLRF ALPGVGKPAA VVLPSSKHGL RAAVLCARAA ALAVRVRSGG HSYEGLSYTT 120 ENRVPFAVID LAGLSRVRVD GGSATAWAEA GATLGELYHA VGTSSRTLAF PAGSCSTIGL 180 GGIVSGGGFG LLSRKHGLAA DNVLDAVLID PSGNILTRDT MGDDVFWAIR GGGGGSWGVV 240 YAWKLRLVPV PDTITVFTAS RTGPVELVAG LVHRWQFVGP NLPDDFYLSV YLPTGGGSSP 300 DSNVSVSFTG QVLGPKRRAM AALRRSFPEL GLAASELAET SWLDATAQFA GLATAADLPD 360 RRLGSKQYSK GKSDYVRSPI PLHTMAGIVR YLATGPPEGG CVILDPYGGA MARVGSGATP 420 FPHRAGTLYG VQYQVYWDED AGEAAPVGWL RSLHSFMAPH VSRAPRGAYV NYLDLDLGVN 480 DWTVAAGGSS EAAVARARAS WGAAYFGDNF DRLVRAKTAV DPGNVFNNAQ SIPPLVNV* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 3.0e-10 | 71 | 275 | 212 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 6.0e-12 | 78 | 217 | 141 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam08031 | BBE | 1.0e-15 | 468 | 533 | 66 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAZ05659.1 | 0 | 11 | 535 | 8 | 528 | hypothetical protein OsI_27886 [Oryza sativa Indica Group] |
| RefSeq | NP_001061035.1 | 0 | 11 | 535 | 8 | 528 | Os08g0158200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001140781.1 | 0 | 4 | 535 | 1 | 548 | hypothetical protein LOC100272856 [Zea mays] |
| RefSeq | XP_002445086.1 | 0 | 3 | 535 | 2 | 559 | hypothetical protein SORBIDRAFT_07g003920 [Sorghum bicolor] |
| RefSeq | XP_002445088.1 | 0 | 36 | 510 | 43 | 519 | hypothetical protein SORBIDRAFT_07g003940 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3d2j_A | 0 | 14 | 537 | 6 | 521 | A Chain A, Structure Of Mouse Golgi Alpha-1,2-Mannosidase Ia Reveals The Molecular Basis For Substrate Specificity Among Class I Enzymes (Family 47 Glycosidases) |
| PDB | 3d2h_A | 0 | 14 | 537 | 6 | 521 | A Chain A, Structure Of Mouse Golgi Alpha-1,2-Mannosidase Ia Reveals The Molecular Basis For Substrate Specificity Among Class I Enzymes (Family 47 Glycosidases) |
| PDB | 3d2d_A | 0 | 14 | 537 | 6 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Reticuline |
| PDB | 4ec3_A | 0 | 32 | 537 | 3 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
| PDB | 3gsy_A | 0 | 32 | 537 | 3 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| berberine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| dehydroscoulerine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| sanguinarine and macarpine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
