| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si015253m |
| Family | AA7 |
| Protein Properties | Length: 353 Molecular Weight: 38312.6 Isoelectric Point: 4.7986 |
| Chromosome | Chromosome/Scaffold: 6 Start: 19862737 End: 19868018 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 2 | 119 | 6.7e-29 |
| AIRVHSGGHSYEGQSYTVDGAVLEDGAPFVVIDLMSLNRVRVDAASATTWVECGATLSKVYYAGAASSVSSSSMVNGSSSSLAFTAGLCSTVSVGGHISG SGFGLLLRKFMLTVDNVL | |||
| Full Sequence |
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| Protein Sequence Length: 353 Download |
| MAIRVHSGGH SYEGQSYTVD GAVLEDGAPF VVIDLMSLNR VRVDAASATT WVECGATLSK 60 VYYAGAASSV SSSSMVNGSS SSLAFTAGLC STVSVGGHIS GSGFGLLLRK FMLTVDNVLL 120 PPLPGQLIPV PGTVMVFTLK REGSVDAIAR LVHWWPSVGP ALPDEFYLSI FLMMGGSLQE 180 DDNVTVFPEL DLVEMELSEM SWVKSAMQFA RLSSVEELTS RVSKMKYYGT NKSDYVKQPI 240 QRDALAEILR YLSTGPVGYS VQYGITWKAG KDDGEAGMTW LRTLYEYMAL HVSYDPRAAY 300 VNYIDLDLSM EVAPATTTNA SNFDRLVQAK THIDPANVFN NAQSIPPLER HD* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 5.0e-8 | 1 | 119 | 120 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 1.0e-10 | 3 | 119 | 118 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam08031 | BBE | 8.0e-13 | 299 | 346 | 50 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD03275.1 | 0 | 1 | 349 | 99 | 548 | putative berberine bridge enzyme [Oryza sativa Japonica Group] |
| DDBJ | BAF23509.2 | 0 | 1 | 349 | 103 | 552 | Os08g0343600 [Oryza sativa Japonica Group] |
| GenBank | EAZ05659.1 | 0 | 1 | 348 | 98 | 528 | hypothetical protein OsI_27886 [Oryza sativa Indica Group] |
| RefSeq | NP_001061035.1 | 0 | 1 | 349 | 98 | 529 | Os08g0158200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001061595.1 | 0 | 1 | 349 | 107 | 556 | Os08g0343600 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4ec3_A | 0 | 3 | 352 | 78 | 504 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
| PDB | 3gsy_A | 0 | 3 | 352 | 78 | 504 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2j_A | 0 | 3 | 352 | 97 | 523 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2h_A | 0 | 3 | 352 | 97 | 523 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2d_A | 0 | 3 | 352 | 97 | 523 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Reticuline |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| berberine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| dehydroscoulerine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| sanguinarine and macarpine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG380703 | 209 | 187 | 348 | 1.4013e-45 |
| HX082799 | 198 | 199 | 349 | 1.99993e-41 |
| HX082780 | 185 | 212 | 349 | 4e-35 |
| DT634895 | 281 | 126 | 345 | 1e-34 |
| HO777438 | 453 | 1 | 348 | 1e-32 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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