y
Basic Information | |
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Species | Setaria italica |
Cazyme ID | Si015778m |
Family | AA7 |
Protein Properties | Length: 568 Molecular Weight: 60648.6 Isoelectric Point: 7.9775 |
Chromosome | Chromosome/Scaffold: 6 Start: 4394562 End: 4396265 |
Description | FAD-binding Berberine family protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
AA7 | 68 | 537 | 0 |
GAPKPAAVVVPASLGELRAAVRCARAAGLVVRIRSGGHSYEGLSYTTEDRNAFAVVDLAALGRVRVDADADGGGATAWVESGATLGQVYYAVAASSPTLA FSAGSCPTVGSGGHIAGGGFGLLSRKYGLAGDNVVDAVLVDAGGRVLDRAAMGEDVFWAIRGGGGGTWGAVYAWRVQLRPVPERVTAFVANRRGTVESVA RLVSTWQHVAPWLPDEFYVSAFVGAGLPESDRTGISVTFKGLYLGPRHEALQILTARFPEIGLSDLNPREMSWIESVVFFSGLPEGSSVSDLTDRVLHKK NYFKAKSDYVHRPMPLDQLIRAVEVLSKQPKAYVILDPYGGAMDGFKSSDLPFPHREGNIHGIQYLIEWTADEDGRRDEYMDWLRHFYDLMGAYVPKNPR TAYINYMDLDLGTNNWSSDRRSNIISKIPNPEVEAARAWGERYFLGNYDRLVRAKTMIDPDNVFRNAQSI |
Full Sequence |
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Protein Sequence Length: 568 Download |
MAALALFSLV LRLFAVQAVG GGDGSGSGSS LTACLAAAGV RNVTARGSPA YDAALRVSIQ 60 NLRFAGAGAP KPAAVVVPAS LGELRAAVRC ARAAGLVVRI RSGGHSYEGL SYTTEDRNAF 120 AVVDLAALGR VRVDADADGG GATAWVESGA TLGQVYYAVA ASSPTLAFSA GSCPTVGSGG 180 HIAGGGFGLL SRKYGLAGDN VVDAVLVDAG GRVLDRAAMG EDVFWAIRGG GGGTWGAVYA 240 WRVQLRPVPE RVTAFVANRR GTVESVARLV STWQHVAPWL PDEFYVSAFV GAGLPESDRT 300 GISVTFKGLY LGPRHEALQI LTARFPEIGL SDLNPREMSW IESVVFFSGL PEGSSVSDLT 360 DRVLHKKNYF KAKSDYVHRP MPLDQLIRAV EVLSKQPKAY VILDPYGGAM DGFKSSDLPF 420 PHREGNIHGI QYLIEWTADE DGRRDEYMDW LRHFYDLMGA YVPKNPRTAY INYMDLDLGT 480 NNWSSDRRSN IISKIPNPEV EAARAWGERY FLGNYDRLVR AKTMIDPDNV FRNAQSIPPL 540 GGPGMTRSLP PAAISPKVTC NGSTNDS* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG0277 | GlcD | 3.0e-16 | 80 | 540 | 468 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
pfam08031 | BBE | 2.0e-16 | 469 | 538 | 70 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
pfam01565 | FAD_binding_4 | 3.0e-18 | 80 | 215 | 136 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. |
Gene Ontology | |
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GO Term | Description |
GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
GO:0016491 | oxidoreductase activity |
GO:0050660 | flavin adenine dinucleotide binding |
GO:0055114 | oxidation-reduction process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAC65012.1 | 0 | 31 | 567 | 31 | 562 | putative berberine bridge enzyme [Oryza sativa Japonica Group] |
GenBank | EAZ05661.1 | 0 | 31 | 567 | 31 | 562 | hypothetical protein OsI_27888 [Oryza sativa Indica Group] |
GenBank | EAZ41578.1 | 0 | 244 | 567 | 42 | 362 | hypothetical protein OsJ_26112 [Oryza sativa Japonica Group] |
RefSeq | XP_002445090.1 | 0 | 1 | 567 | 1 | 512 | hypothetical protein SORBIDRAFT_07g003960 [Sorghum bicolor] |
RefSeq | XP_002533924.1 | 0 | 28 | 540 | 28 | 535 | d-lactate dehydrogenase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 4ec3_A | 0 | 27 | 542 | 4 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
PDB | 3gsy_A | 0 | 27 | 542 | 4 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
PDB | 3d2j_A | 0 | 27 | 542 | 23 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
PDB | 3d2h_A | 0 | 27 | 542 | 23 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
PDB | 3d2d_A | 0 | 27 | 542 | 23 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Reticuline |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
berberine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
dehydroscoulerine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
sanguinarine and macarpine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |