| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si039535m |
| Family | GH32 |
| Protein Properties | Length: 583 Molecular Weight: 63542.4 Isoelectric Point: 6.2007 |
| Chromosome | Chromosome/Scaffold: 9 Start: 6206307 End: 6208274 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 47 | 363 | 0 |
| HFQPPKHWINDPNGVMYYKGVYHLFYQYNPKGAVWGNIVWAHAVSTDLVDWIMLPPAIYPTAPFDVNGCWSGSATVLPDGTPVIMYTGIDGENRQVQNVA YPKDLSDPYLREWVKPDYNPVIAPGPGMNATAFRDPTTAWQGPDGLWRLVIGTKDNHRGLAVLYRSRDFKRWAPAKRALHSGDTGMWECPDFYPVTTSPA VGSGGGGSGNGTKHVLKVSLDLTRFEYYTFGEYDHATDTYVPDAALADGNDGLRYDYGNFYASKTFLDTAKQRRVLWGWANESDSATADIRKGWAGVQAI PRKIWLSSDGKQLVQWP | |||
| Full Sequence |
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| Protein Sequence Length: 583 Download |
| MAIRAWTWVV LLLLALLPSC RAAEAPSPQP SADPELAVDD GLRTGYHFQP PKHWINDPNG 60 VMYYKGVYHL FYQYNPKGAV WGNIVWAHAV STDLVDWIML PPAIYPTAPF DVNGCWSGSA 120 TVLPDGTPVI MYTGIDGENR QVQNVAYPKD LSDPYLREWV KPDYNPVIAP GPGMNATAFR 180 DPTTAWQGPD GLWRLVIGTK DNHRGLAVLY RSRDFKRWAP AKRALHSGDT GMWECPDFYP 240 VTTSPAVGSG GGGSGNGTKH VLKVSLDLTR FEYYTFGEYD HATDTYVPDA ALADGNDGLR 300 YDYGNFYASK TFLDTAKQRR VLWGWANESD SATADIRKGW AGVQAIPRKI WLSSDGKQLV 360 QWPVAEIESL RGAHANVTDT LVAGGSHFEV TGLASAAQAD VEASFQVMDL DKAEPFDPAW 420 RGADAQTVCA ARGADARGGV GPFGLWALAS DEHGERTAVF FRVFKGGDGG KHVVLMCNDP 480 SMSSHADDLY KPTFAGFVDV DIAQTGGKIA LRTLIDHSVV ESFGAHGKTC ILSRVYPTKA 540 VGDKARLYVF NNGESDVKVT HLNAYEMRSA KITSDAVEPT KR* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 1.0e-71 | 34 | 390 | 376 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-96 | 53 | 366 | 327 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-153 | 47 | 363 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-179 | 47 | 527 | 491 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAO21213.1 | 0 | 34 | 568 | 49 | 579 | cell wall invertase [Musa acuminata] |
| GenBank | AAQ24870.1 | 0 | 5 | 575 | 9 | 585 | cell wall invertase 3 [Oryza sativa Indica Group] |
| EMBL | CAD40590.2 | 0 | 5 | 575 | 9 | 582 | OJ000126_13.7 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001052747.1 | 0 | 5 | 575 | 9 | 585 | Os04g0413200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002466502.1 | 0 | 1 | 575 | 1 | 577 | hypothetical protein SORBIDRAFT_01g008910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 37 | 573 | 3 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 43 | 573 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 43 | 573 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 43 | 573 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 43 | 573 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
