| Basic Information | |
|---|---|
| Species | Thellungiella halophila |
| Cazyme ID | Thhalv10005878m |
| Family | AA7 |
| Protein Properties | Length: 537 Molecular Weight: 60603.5 Isoelectric Point: 9.2945 |
| Chromosome | Chromosome/Scaffold: 19 Start: 36664 End: 38745 |
| Description | cytokinin oxidase/dehydrogenase 6 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 66 | 257 | 3.6e-25 |
| NRYQLLPLAVLHPKSVSDIASAIRYIWMMGHHSQLTVAARGRGHSLQGQAQTRHGIVIHMESLQPQKLQVHSVGAPTPYVDVSGGELWINILHETLKSGL APKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEILNCSERQNSDLFHGVLGGLGQFGIITRARIALEPAPAMVKWMRV | |||
| Full Sequence |
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| Protein Sequence Length: 537 Download |
| MSYLHACLIG KRNMLIIRSF TTLLLTCIAF RLACCFSSSI SPLKALPLVG HLEFEDIHHA 60 SKDFGNRYQL LPLAVLHPKS VSDIASAIRY IWMMGHHSQL TVAARGRGHS LQGQAQTRHG 120 IVIHMESLQP QKLQVHSVGA PTPYVDVSGG ELWINILHET LKSGLAPKSW TDYLHLTVGG 180 TLSNAGISGQ AFRHGPQISN VHQLEVVTGK GEILNCSERQ NSDLFHGVLG GLGQFGIITR 240 ARIALEPAPA MVKWMRVLYL DFSAFAKDQE RLISADKRFD YIEGFVIINR TGLLNNWRLS 300 FTPEDPLQAS QFKSDGRTLY CLEVGKYFKM DDNKDVMNQE VKEWLSELSY VSSTLFSTEV 360 TYEEFLDRVH VSEKKLRSKG QWEVPHPWLN LLVPRSRVND FAKGVFGSIL TDTSNGPVIV 420 YPVNKSKWDN RTSAVTPEEE IFYLVAILTS AVPGSTGKDG VEHILKRNQR ILEFSQAQGF 480 GLKQYLPHYT TRREWRSHFG PKWEDFVRRK SRYDPLAMLA PGQRIFEKAQ WISHSS* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01679 | bact_FAD_ox | 3.0e-9 | 72 | 306 | 235 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| COG0277 | GlcD | 8.0e-23 | 65 | 523 | 470 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 6.0e-25 | 72 | 217 | 147 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 4.0e-148 | 249 | 526 | 282 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 11 | 529 | 526 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAF00384.1 | 0 | 14 | 529 | 1 | 516 | cytokinin oxidase -like protein [Arabidopsis thaliana] |
| RefSeq | NP_191903.3 | 0 | 1 | 529 | 1 | 529 | CKX6 (CYTOKININ OXIDASE/DEHYDROGENASE 6); cytokinin dehydrogenase [Arabidopsis thaliana] |
| Swiss-Prot | Q9LY71 | 0 | 14 | 529 | 1 | 500 | CKX6_ARATH RecName: Full=Cytokinin dehydrogenase 6; AltName: Full=Cytokinin oxidase 6; Short=CKO6; Short=AtCKX6; Short=AtCKX7; Flags: Precursor |
| RefSeq | XP_002304773.1 | 0 | 14 | 529 | 1 | 513 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002332424.1 | 0 | 1 | 529 | 1 | 526 | cytokinin oxidase [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 60 | 529 | 50 | 523 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 2exr_A | 0 | 60 | 529 | 50 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 43 | 527 | 20 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 43 | 527 | 20 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 43 | 527 | 20 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| ES837714 | 291 | 165 | 455 | 0 |
| DV137371 | 273 | 206 | 478 | 0 |
| DY963441 | 302 | 50 | 351 | 0 |
| HO781924 | 497 | 37 | 526 | 0 |
| JK618028 | 278 | 69 | 346 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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